A kind of immobilized cultivation device and cultivation method

A technology of immobilized culture and culture medium, which is applied in the field of microalgae culture, and can solve the problems of low yield of immobilized microalgae culture and poor supply of culture medium

Active Publication Date: 2017-07-07
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] When cultivating by the above-mentioned immobilized cultivation device, due to the gas dissolved in the culture solution, when the culture solution is supplied, bulging will occur between the substrate 1 and the infusion layer 2, the infusion layer 2 and the carrier 3. Bubble phenomenon, so that the supply of culture solution is not smooth, the microalgae cell layer 4 attached to the carrier 3 is suspended in the culture solution supply layer, resulting in low yield of immobilized culture of microalgae

Method used

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  • A kind of immobilized cultivation device and cultivation method
  • A kind of immobilized cultivation device and cultivation method
  • A kind of immobilized cultivation device and cultivation method

Examples

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specific Embodiment approach

[0046] Hereinafter, the embodiments of the present invention set up comparative examples, examples and experimental examples to illustrate the present invention. These examples are only examples proposed to specifically illustrate the present invention, and those skilled in the art can understand that the scope of the present invention is not limited by these examples, comparative examples and experimental examples.

[0047] Wherein, it should be noted that, in order to objectively evaluate the technical effect of the embodiment of the present invention, the control example and the embodiment of the present invention have the same substrate structure except that the other conditions are the same. For example: the species of microalgae inoculated are all Chlorella reinhardtii, the culture solution is BG11 culture solution containing various nutrients such as nitrogen and phosphorus, and the immobilized microalgae culture area is 0.3 square meters. The immobilized culture device ...

Embodiment 1

[0051] The substrate used in Example 1 is provided with a plurality of grooves on the contact surface of the infusion layer, and the output end of the culture solution of each groove communicates with the outside of the immobilized culture device. The cross-sectional shape perpendicular to the extension direction of the groove is triangular tooth shape, the depth of a single tooth is about 0.5 cm, and the spacing is 0.8 cm. During the breeding process, the immobilized breeding surface accepts the same as the control example from 7:00 to 17:00 every day. light, cultivated for seven days.

Embodiment 2

[0053] Through holes are evenly distributed on the contact surface between the base plate and the infusion layer of the immobilized culture device used in Example 2. The diameter of the through holes is 0.8 cm, and the center-to-center distance is 2.4 cm. During the breeding process, the immobilized culture surface received the same light as the control example from 7 o'clock to 17 o'clock every day, and cultured for seven days.

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Abstract

The invention relates to the field of microalgae cultivation, in particular to an immobilized cultivation device and a cultivation method. The method can reduce the bubbling phenomenon during the conveying process of the culture solution, improve the supply stability of the culture solution, and further increase the immobilized cultivation output of microalgae. It overcomes the defects in the prior art that when the culture solution is transported, the air bubbles cannot be discharged so that bubbling occurs between the substrate and the infusion layer, resulting in poor supply of the culture solution and low yield of immobilized culture of microalgae. An embodiment of the present invention provides a fixed culture device, including: a base plate and a carrier, and an infusion layer arranged between the base plate and the carrier; wherein, the contact surface between the base plate and the infusion layer is provided with exhaust part, when the infusion layer supplies the culture solution to the carrier, the air bubbles in the culture solution are discharged to the outside of the immobilized culture device through the exhaust part.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to an immobilized cultivation device and a cultivation method. Background technique [0002] Immobilized cultivation technology can be applied to large-scale cultivation of various microorganisms, and has become the only way to realize the large-scale proliferation of microalgae. The immobilized culture device usually consists of a substrate 1, an infusion layer 2 and a carrier 3, see figure 1 , and the substrate 1, the infusion layer 2 and the carrier 3 are connected to each other through the surface in turn, the substrate 1 is used for supporting, and the infusion layer 2 is used to provide culture fluid to the surface of the carrier, and the substrate attached to the surface of the carrier 3 The microalgae cell layer 4 is immobilized cultured. [0003] When cultivating by the above-mentioned immobilized cultivation device, due to the gas dissolved in the culture solution, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N1/12
Inventor 张凯卢彦兴冯倩桑俊宝陈传红
Owner ENN SCI & TECH DEV
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