Screening method for 43 artificial synthetic pigments in aquatic product

Abstract

The invention relates to a screening method for 43 artificial synthetic pigments in an aquatic product. The method comprises the following steps: screening by virtue of quadrupole tandem time-of-flight mass spectrometry of liquid chromatogram; calling an established mass spectrometry screening database to automatically scan and retrieve in a molecular matching mode; through a C18 analytic column, carrying out gradient elution by taking an acetonitrile solution containing 0.1% of formic acid in a 5mmol/L ammonium acetate aqueous solution as a moving phase, wherein the flow rate is 0.3ml/minute; and detecting under a negative ion mode by using an electrospray ionization source, extracting a suspicious sample, purifying the sample by virtue of a matrix dispersion method, carrying out liquid chromatographic separation, quantitatively determining by virtue of an external standard method, and verifying. The screening method has the advantages that the detection method is simple and quick to operate and high in sensitivity. By adopting the matrix dispersion method to purify the sample, the interference of matrix components is effectively reduced. Misjudgment events such as false positive events are greatly reduced by virtue of the qualitative function of the quadrupole tandem time-of-flight mass spectrometry, so that the monitoring ability of the detection mechanism on the risk of the aquatic product is extremely enhanced.

Description

technical field [0001] The invention relates to a detection method for food additives, in particular to a screening method for 43 kinds of artificially synthesized pigments in aquatic products. Background technique [0002] In recent years, illegal addition and abuse of pigments in aquatic products have emerged in an endless stream. Therefore, related detection methods have also been concerned and studied. Synthetic pigments are mainly used in industrial production such as dyeing wool, leather, silk, etc. due to their bright color and easy coloring. They are all toxic chemicals. However, in order to save costs, some criminals illegally add synthetic pigments, such as azorubin, acid red, Sudan red, etc., to aquatic products, which has caused great harm to people's health and social stability. Therefore, it is necessary to establish a screening method for artificial pigments in aquatic products, and provide technical means for effectively curbing illegal additions of aquatic...

AI Technical Summary

Problems solved by technology

But its disadvantage is that the sample pretreatment is complicated, and it can only be measured and corrected by first-level information. Only liquid chromatography / time of flight is used, so the detection efficiency is not high, the speed is not fast, and the accuracy is not high. Moreover, the above method, It can only be applied to the screening of acid pigments, but not to the screening of basic pigments, so this detection method needs to be further improved

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