Method for detecting glyphosate and aminomethylphosphonic acid residues in transgenic soybeans
A technology of genetically modified soybeans and aminomethylphosphonic acid, which is applied in the direction of measuring devices, instruments, and bulk chemical production, can solve the problems of few research reports on genetically modified soybeans, large influence of matrix interference, and low sensitivity, so as to promote sustainable and healthy development , high sensitivity, wide application prospects
Pending Publication Date: 2022-07-08
舟山出入境检验检疫局综合技术服务中心
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Problems solved by technology
Gas chromatography and gas chromatography-mass spectrometry need to use more reagents to convert target substances into gasifiable substances, and the process is complicated and time-consuming
When using liquid chromatography and liquid chromatography-mass spectrometry, if it is directly extracted and tested on the machine, it will be greatly affected by matrix interference and the sensitivity will not be high
Pre-column derivatization-LC-MS is a common method for detecting glyphosate and aminomethylphosphonic acid in recent years. For water, soil, tea, rice and other substrates, there are relatively few research reports on transgenic soybeans
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Embodiment 1
[0059] A method for detecting glyphosate and aminomethylphosphonic acid residues in genetically modified soybeans, comprising the following steps:
[0060] (1) Configuration standard products:
[0061] Accurately pipette 1.0ml of glyphosate and aminomethylphosphonic acid mixed standard solution (mass concentration 1000mg / L) into a 100ml volumetric flask, dilute to 100ml with pure water, and prepare a 10mg / L mixed standard stock solution. Store at 4°C; accurately pipette the mixed standard solution and dilute with pure water to a series of mixed standards with concentrations of 1ng / ml, 2ng / ml, 5ng / ml, 10ng / ml, 20ng / ml, 50ng / ml and 100ng / ml As the working solution, draw the isotope 1,2-C13N15 glyphosate internal standard solution with a concentration of 1 μg / mL, and store it in a 4°C refrigerator until testing.
[0062] (2) Sample extraction, purification and derivatization:
[0063] Extraction: The soybeans were crushed in a pulverizer and passed through a 60-mesh sieve to ob...
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Abstract
The invention discloses a method for detecting glyphosate and aminomethylphosphonic acid residues in transgenic soybeans, and particularly relates to a detection method for determining the glyphosate and aminomethylphosphonic acid residues in the transgenic soybeans by utilizing a pre-column derivatization-UPLC-MS / MS-internal standard method. UPLC-MS / MS is adopted, determination is carried out in an electrospray ionization (ESI < + >) and multi-reaction monitoring mode, quantitative analysis is carried out by using an isotope internal standard method, and rapid separation and accurate qualitative and quantitative determination of glyphosate and aminomethylphosphonic acid in transgenic soybeans are realized. The method is high in sensitivity, good in recovery rate, strong in qualitative ability and high in resolution ratio, the pretreatment process is simple to operate, and the requirements of on-line automatic detection can be met, so that the purposes of green, efficient and accurate qualitative and quantitative analysis are achieved. The method is easy to operate, short in time consumption, good in extraction rate, capable of effectively reducing interference of complex matrix components, low in detection limit and suitable for daily detection work of glyphosate and aminomethylphosphonic acid residues in transgenic soybeans.
Description
technical field [0001] The invention relates to a method for detecting the residues of glyphosate and its main metabolite aminomethylphosphonic acid in transgenic soybeans, and belongs to the technical field of agricultural product detection. Background technique [0002] Soybean is a kind of leguminous plant that is rich in high-quality protein, has high nutritional value, and is one of the important sources of protein in human daily diet. Affected by traditional eating habits, soybean oil is also a daily necessity. The domestic supply of ordinary soybeans is in short supply, so it is necessary to import a large amount of genetically modified soybeans from overseas to meet the market demand. Therefore, my country has become the country with the largest import volume and crushing volume of genetically modified soybeans in the world. [0003] Transgenic soybean is to recombine and express the exogenous gene (artificially isolated or modified gene) with the genomic DNA of ord...
Claims
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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/7266G01N30/8675G01N2030/045G01N2030/067Y02P20/55
Inventor 君辉沈飚
Owner 舟山出入境检验检疫局综合技术服务中心
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