Fish gill-rot disease pathogenic flavobacterium johnsoniae attenuated live vaccine and construction method thereof

A technology of Flavobacterium johniii live attenuated vaccine, which is applied in the field of fish gill rot pathogen Flavobacterium johniii live attenuated vaccine and its construction, which can solve the problems of wide range of disease, discouragement of fishermen's production enthusiasm, and long epidemic time. , to achieve broad market prospects, good application value, and the effect of small immune doses

Active Publication Date: 2015-07-22
湖北宜水环保科技有限公司
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Problems solved by technology

Among them, bacterial gill rot caused by Flavobacterium is one of the most common diseases in aquaculture. Serious economic losses have hit fishermen's production enthusiasm, brought about quality and safety problems of aquatic products such as drug residues, and restricted the sustainable and healthy development of my country's aquacul

Method used

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  • Fish gill-rot disease pathogenic flavobacterium johnsoniae attenuated live vaccine and construction method thereof
  • Fish gill-rot disease pathogenic flavobacterium johnsoniae attenuated live vaccine and construction method thereof
  • Fish gill-rot disease pathogenic flavobacterium johnsoniae attenuated live vaccine and construction method thereof

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Embodiment

[0022] 1. Induction of attenuated mutant strains of Flavobacter johniiii

[0023] The pathogenic Flavobacterium johnii strain (M168) was isolated from freshwater fish suffering from gill rot, and after purification and cultivation by streaking on a plate, the concentration of the bacterial solution was adjusted to 10 with sterilized isotonic saline. 7 CFU / mL spare. Add 0.005, 0.01, 0.02, 0.03, 0.06, 0.13, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 64, 128 μg / mL streptomycin to the M-H broth medium, and prepare different medium with streptomycin concentration. Inoculate 10 μL of the above-prepared bacterial suspension into 2 mL broth medium containing 0.005 μg / mL streptomycin, incubate at 28°C for 24 h, and if there is bacterial growth, transfer it to a medium containing 0.01 μg / mL streptomycin If bacteria grow after incubation at 28°C for 24 hours, transfer to the next concentration of streptomycin medium. If no bacterial growth is found, continue to culture in the previous concentrat...

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Abstract

The invention discloses a fish gill-rot disease pathogenic flavobacterium johnsoniae attenuated live vaccine and a construction method thereof. Wild-type flavobacterium johnsoniae is subjected to continuous passage induction mutation to make the toxicity weakened by streptomycin, and a flavobacterium johnsoniae attenuated mutant strain M170 is obtained; the live vaccine prepared by using the attenuated strain immunizes grass carp, mandarin fish and the like through injection and soaking, the immune protective rates all can reach 73.1% or more, and the immune protection period reaches 8 months or more; the live vaccine has the advantages of small immunizing dose, high titer, high protection rate, long protection period and the like, is used for immunization by the soaking way, can prevent occurrence of bacterial gill-rot disease of grass carp, mandarin fish and the like, and has good application value and broad market prospect.

Description

Technical field [0001] The present invention involves a fish rotten gill disease. FlavoBacterium Johnsoniae ) Poisoning live vaccine and construction method. Background technique [0002] China is a major aquaculture country, and the production of aquaculture accounts for 70%of the global total output.With the rapid development of my country's aquaculture industry, the expansion of the scale of breeding and the increase in unit output, the disease problems are increasingly prominent.Among them, bacterial rotten gill diseases caused by mobilization are one of the most common diseases in aquaculture. It has the characteristics of highly endangered breeding varieties, high incidence, wide range of onset, and long epidemic time. It brings to my country's aquaculture industry each year. Serious economic losses have cracked down on the production enthusiasm of fishermen, bringing the quality and safety of aquatic products such as drug residues, and restricting the sustainable and healt...

Claims

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Application Information

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IPC IPC(8): A61K39/02C12N1/21C12N1/20A61P31/04C12R1/20
Inventor 李宁求林强付小哲刘礼辉郭慧芝吴淑勤
Owner 湖北宜水环保科技有限公司
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