A kind of construction method of lotus regeneration system
A technology for constructing a method and a system, applied in the field of plant tissue culture, can solve the problems of high pollution rate and high lethality rate, and achieve the effects of low pollution rate, guaranteed sources, and widening the selection range.
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Embodiment 1
[0031] A method for constructing a lotus regeneration system is as follows:
[0032] (1) The preparation of the medium, including the basic medium and the components of the medium at each stage and the weight per liter are:
[0033] 1) Basic medium: MS;
[0034] 2) Sterile seedling medium: MS+6-BA 1mg / L+NAA 0.5mg / L+sucrose 30g / L, agar 8g / L, pH=5.8;
[0035] 3) Callus induction medium: MS+TDZ 0.1-0.2mg / L+NAA 0.7-0.8mg / L+sucrose 50g / L, agar 8g / L, pH=5.6,
[0036] 4) Adventitious bud induction medium: MS+6-BA 50μM+sucrose 30g / L, agar 8g / L, pH=5.6,
[0037] 5) Proliferation medium: MS+6-BA 1mg / L+NAA 0.5mg / L+sucrose 30g / L, agar 8g / L, pH=5.6
[0038] 6) Rooting medium: MS+IBA 1-2mg / L+sucrose 30g / L, agar 8g / L, pH=5.6
[0039] (2) Sterilization and shell breaking:
[0040] Test 1: Change the order of shelling and sterilization, and set up the following two treatment groups:
[0041] Treatment group 1: Select the round and plump lotus seeds of "Guangchang Bailian" and disinfect them with 75% alcohol ...
Embodiment 2
[0072] (1) The preparation of the medium, including the basic medium and the components of the medium at each stage and the weight per liter are:
[0073] 1) Basic medium: MS;
[0074] 2) Sterile seedling medium: MS+6-BA 1mg / L+NAA 0.5mg / L+sucrose 30g / L, agar 8g / L, pH=5.8;
[0075] 3) Callus induction medium: MS+TDZ 0.1-0.2mg / L+NAA 0.7-0.8mg / L+sucrose 50g / L, agar 8g / L, pH=5.6,
[0076] 4) Adventitious bud induction medium: MS+6-BA 50μM+sucrose 30g / L, agar 8g / L, pH=5.6,
[0077] 5) Proliferation medium: MS+6-BA 1mg / L+NAA 0.5mg / L+sucrose 30g / L, agar 8g / L, pH=5.6
[0078] 6) Rooting medium: MS+IBA 1-2mg / L+sucrose 30g / L, agar 8g / L, pH=5.6
[0079] (2) In March 2014, the round and plump seeds of "Guangchang Bailian" were selected to be sterilized with 75% alcohol for 30 seconds, rinsed with sterile water once, soaked in 3% sodium hypochlorite for 8 minutes, and rinsed with sterile water for 3 times. spare.
[0080] (3) Cut the lotus seeds with sterile pruning shears, take out the lotus core as ...
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