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Method for rapidly warning mildewing of tobacco and tobacco products

A technology for tobacco products and tobacco, which is applied in the field of rapid early warning of mildew in tobacco and tobacco products. It can solve the problems of not being able to play the role of early warning of tobacco mildew and relatively high requirements for instrument conditions, so as to reduce the time spent and improve the appearance. Features, effects of easy operation

Inactive Publication Date: 2015-08-05
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

[0006] Existing tobacco mildew monitoring methods include microbiological testing, such as mold counting methods, which require a 5-day experiment cycle; chemical methods, such as gas chromatography-mass spectrometry for the determination of ergosterol in moldy tobacco, require samples to be derivatized before Treatment: Determination of ergosterol in mildewed tobacco by high performance liquid chromatography-tandem mass spectrometry. This method needs to grind and extract tobacco samples, and has relatively high requirements for instrument conditions, and ergosterol is more suitable for use in tobacco mildew. In this case, it cannot play a good early warning role of tobacco mildew

Method used

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  • Method for rapidly warning mildewing of tobacco and tobacco products
  • Method for rapidly warning mildewing of tobacco and tobacco products
  • Method for rapidly warning mildewing of tobacco and tobacco products

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Embodiment 1

[0027] A method for rapid early warning of mildew in tobacco and tobacco products, comprising the following steps:

[0028] The first step, tobacco mildew

[0029] 1. Weigh 0.1~0.3g of tobacco fragments, put them into a 20mL headspace bottle, and prepare several samples in total. These samples were analyzed for VOCs and served as non-mildewed tobacco controls. Then do not add a cover, be placed in the constant temperature and humidity box to impel the mildewed tobacco to produce mildew, and the temperature in the constant temperature and humidity box is 35 ℃, and the relative humidity is 85%.

[0030] 2. On the 5th, 7th, 9th, 11th, 12th, 13th, 14th, 15th, 16th, and 17th days, take samples, take the samples out of the constant temperature and humidity chamber, cover them, and analyze the volatile organic compounds in the samples.

[0031] 3. On the 13th day of the experiment, no mildew phenomenon was observed on the tobacco surface, and 1-octen-3-ol could be qualitatively det...

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Abstract

The invention discloses a method for rapidly warning mildewing of tobacco and tobacco products and belongs to the technical field of rapid warning of mildewing of tobacco and tobacco products. The method includes performing sample injection by means of headspace solid-phase microextraction, determining a volatile organic compound 1-octene-3-alcohol featuring tobacco mildewing generation qualitatively and quantitatively rapidly by a gas chromatography / mass spectrometry external reference method, and warning tobacco mildewing in an initial stage when tobacco mildewing cannot be recognized by naked eyes so as to prevent loss caused by tobacco mildewing. Compared with an existing tobacco mildewing determining method by determining ergosterol by a gas chromatography or liquid chromatography-tandem mass spectrometry method in the tobacco industry, the method for rapidly warning mildewing of the tobacco and the tobacco products has the advantages that organic solvent extraction is not required in sample pretreatment, and accordingly, environmental friendliness is achieved; the tobacco or the tobacco products are placed in a headspace bottle directly and appropriate equilibrium temperature is set, thus, the method is rapid, convenient and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of rapid early warning of mildew of tobacco and tobacco products, and in particular relates to a method for rapid early warning of mildew of tobacco and tobacco products. Background technique [0002] Mildew has a serious impact on the quality and safety of cigarette products. During alcoholic storage, processing and transportation of tobacco and tobacco products, if the ambient temperature and humidity conditions are suitable, bacteria or fungal microorganisms will grow in large numbers, causing mildew in tobacco and tobacco products, resulting in Musty, peculiar smell. As the degree of mildew increases, the quality of tobacco and tobacco products will be reduced until they lose their use value, and the carcinogenic toxicity of mold products will also endanger the health of consumers. Aspergillus niger Aspergillus niger (AN) 、 Aspergillus flamboyant Aspergillus ornatus (AO) 、 Penicillium chrysogenum ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 刘彤陈欢韩书磊付亚宁侯宏卫胡清源
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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