Roundworm mi RNA inhibitor and application thereof

A technology of inhibitors and roundworms, applied in the field of biological agents, can solve problems such as drug residues, achieve the effects of reducing abundance, reducing infection rates, and avoiding drug resistance problems of parasites

Active Publication Date: 2015-08-26
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a roundworm miRNA inhibitor aiming at the problem of parasite drug re

Method used

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  • Roundworm mi RNA inhibitor and application thereof
  • Roundworm mi RNA inhibitor and application thereof
  • Roundworm mi RNA inhibitor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Ascaris miRNA inhibitor of the present invention inhibits effect experiment on ascaris:

[0022] 1. Preparation of Tertiary Larvae of Ascaris suum

[0023] Collect Ascaris suum adult females from the small intestine of naturally infected slaughtered pigs, dissect and remove about 1 / 2 of the fork end of the uterus, take out the eggs, put them into a mortar, and wash them with sterilized PBS solution (Na 2 HPO 4 12H 2 O 9.670g; KH 2 PO 4 1.429g; NaCl 2.125g; H 2 O 500ml) was slightly ground in a mortar, washed with PBS solution and 1.5% formalin solution, centrifuged twice, discarded the supernatant, put the precipitate into a petri dish, and cultivated in a 28°C incubator for 28 days. The third-stage larvae of Ascaris suum were obtained by shelling with 7.5% hypochlorous acid.

[0024] 2. Preparation of culture medium

[0025] Prepare DMEM medium containing 50% fetal bovine serum, and add penicillin (1000U / ml), streptomycin (1mg / ml) and amphotericin (10μg / ml) to...

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Abstract

The invention provides a roundworm mi RNA inhibitor. The nucleotide sequence of the inhibitor is: UCUACCCGAGAAUCGAACAGUC. Through combination with asu-miR-100 b, the inhibitor obviously reduces the abundance of asu-miR-100 b, significantly influences on the growth of the third-stage larvae of ascaris sum and reduces the infection rate. The asu-miR-100 b inhibitor expressed by the whole growth period of roundworm can be applied to influence the growth or functions related to its infectivity of the third-stage larvae of ascaris sum and ascaris lumbricoides.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to an ascaris miRNA inhibitor and an application thereof. Background technique [0002] Ascaris suum ( Ascaris suum ) is one of the common parasitic nematodes in pigs, which belongs to Ascaridae and lives in the small intestine of pigs. Ascariasis suum is prevalent widely, which greatly endangers the development of pig industry, especially in pig farms with poor sanitation, the infection rate is very high. The main manifestations of sick pigs are poor growth and development, and severe cases can cause growth and development stagnation, or even death. The long-term overuse of anti-helminth drugs has caused the problem of drug resistance in parasites distributed globally and the problem of drug residues in animal products and the environment. Therefore, it is imminent to speed up the research on Ascaris suum and find new ways to effectively control Ascar...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K31/7088A61P33/10
Inventor 徐民俊邵长春朱兴全冯胜勇
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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