System and method for partition electrospinning of multiple materials
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A multi-material, electrospinning technology, which is applied in the fields of electrospinning and biomanufacturing, can solve problems such as restricting the application of electrospinning technology and uncontrollable receiving fibers.
Active Publication Date: 2015-08-26
无锡微控医疗科技有限公司
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Problems solved by technology
The electrospinning method is simple and easy to operate, but the disadvantage of uncontrollable receiving fibers seriously restricts the application of electrospinning technology in various fields.
Method used
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Embodiment 1）
[0026] Such as figure 2 As shown, the method (embodiment 1) of the present invention that can realize multi-material partition electrospinning comprises the following steps:
[0027] 1) Prepare two 8.8% PVA (polyvinyl alcohol) solutions, both 20ml;
[0028] 2) Take the second-generation human adipose stem cells and centrifuge them at 1000r / min for 5min to prepare a cell content of 4X10 6 cells / ml cell suspension; take the second-generation human fibroblasts and centrifuge at 1000r / min for 5min to prepare a cell content of 4X10 6 cell suspension per ml;
[0029] 3) Mix 5ml of adipose stem cells with a portion of PVA, stir evenly, and put it into the barrel m; put a portion of the PVA solution into the outer channel of the barrel n, and put 5ml of the cell suspension into the barrel n in the inner channel;
[0030] 4) When the conductor is located figure 2 At the position S1, the micropump of the feeding mechanism controls the supply of the electrospinning solution, and t...
Embodiment 2
[0037] This embodiment is basically the same as Embodiment 1, and the special features are as follows: the double nozzles are expanded into three nozzles, and the three nozzles all adopt coaxial nozzles, including the following steps:
[0038] 1) Prepare three 8.8% PVA solutions, each 20ml;
[0039] 2) Prepare solutions of vascular endothelial growth factor (VEGF), fibroblast growth factor (bFGF), and epidermal growth factor (EGF) at a concentration of 2%;
[0040] 3) Put a portion of PVA into the outer channel of the barrel m of the multi-nozzle feeding mechanism, and put a solution of vascular endothelial growth factor (VEGF) into the inner channel of the barrel m of the multi-nozzle feeding mechanism; One part of PVA is loaded into the outer channel of the barrel n of the multi-nozzle feeding mechanism, and the fibroblast growth factor (bFGF) solution is loaded into the inner channel of the barrel n of the multi-nozzle feeding mechanism; one part of PVA Put it into the out...
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Abstract
The invention provides a system and method for partition electrospinning of multiple materials. The system comprises a multi-spraying-head feeding mechanism and the like, wherein the multi-spraying-head feeding mechanism is located above a receiving component with distance adjustable, the receiving component is fixed above a tray through a support and can be replaced, and a gap is reserved between a conductor and the receiving component; the positive electrode of a high-voltage power supply is connected with the positive electrode of each spray head of the multi-spraying-head feeding mechanism, and the negative electrode of the high-voltage power supply is connected with the conductor; the multi-spraying-head feeding mechanism, the tray and a computer control system are connected, and multi-spraying-head feeding switch of the multi-spraying-head feeding mechanism and rotation or movement of the tray are controlled by the computer control system. The partition electrospinning method and cell electrospinning are combined so that different cells can be distributed on an electrostatic spinning fibrous membrane in a controllable mode, and a three-dimensional structure similar to vascular tissue can be obtained after the electrostatic spinning fibrous membrane is curved.
Description
technical field [0001] The invention relates to a system and method capable of realizing multi-material partition electrospinning, and belongs to the field of electrospinning and the technical field of biomanufacturing. Background technique [0002] In recent years, due to the rapid heating up of nanomaterial research, people have aroused strong interest in in-depth research on electrospinning. The nanofibers spun by electrospinning have small diameter and large specific surface area, and have been widely used in biomedical materials, filtration and protection, catalysis, energy, optoelectronics, food engineering, cosmetics and other fields. Electrospinning has become the most important and effective method for preparing nanofibers. The electrospinning method is simple and easy to operate, but the disadvantage of uncontrollable receiving fibers seriously restricts the application of electrospinning technology in various fields. [0003] Electrospinning technology is also w...
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