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Culture medium for rapid propagation in tissue culture of bletilla striata

A culture medium and technology of Bletilla striata group, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., to achieve the effects of large bulbs, improved seed germination rate, and strong plants

Active Publication Date: 2015-09-23
虹越花卉股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing Bletilla striata tissue culture and rapid propagation technology needs to be further improved in terms of seed germination rate, tissue culture seedling rooting and bulb size.

Method used

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  • Culture medium for rapid propagation in tissue culture of bletilla striata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) With medium:

[0024] Each liter of seeding medium consists of the following components: 8g of agar + 30g of sugar + 80ml of coconut water + 0.05mg of 6-BA + 0.25mg of NAA, the rest is MS, pH=5.4.

[0025] Each liter of subculture medium consists of the following components: agar 8g + sugar 30g + coconut water 80ml + activated carbon 0.5g + 6-BA 0.5mg + NAA 0.1mg, the rest is MS, pH=5.4.

[0026] Each liter of rooting colonization medium consists of the following components: Huabao No. 1 2g + Huabao No. 1g + agar 8g + sugar 20g + activated carbon 1g + banana meat 40g + potato mash 20g, pH=5.4.

[0027] 2) Selection of explants: Select robust wild Bletilla striata capsules.

[0028] 3) Sterilization: (1) Rinse the capsule with running water for 30 minutes; (2) Rinse once with sterile water; (3) Disinfect with 75% alcohol for 30 seconds; (4) Soak in 0.1% mercuric solution for 10 minutes; (5) Aseptic Rinse with water 3-5 times.

[0029] 4) Inoculation: Place the ster...

Embodiment 2

[0035] Control group 1, according to the Bletilla striata tissue culture rapid propagation medium reported in the literature, the sowing medium adopts the medium formula reported in the literature, the subculture medium and the rooting medium adopt this patent medium formula, and the medium formula is as follows:

[0036] Each liter of seeding medium consists of the following components: agar 6.5g + sucrose 30g + 6-BA 1.0mg, the rest is 1 / 2MS, pH=5.8.

[0037] Each liter of subculture medium consists of the following components: agar 8g + sugar 30g + coconut water 80ml + activated carbon 0.5g + 6-BA 0.5mg + NAA 0.1mg, the rest is MS, pH=5.4.

[0038] Each liter of rooting colonization medium consists of the following components: Huabao No. 1 2g + Huabao No. 1g + agar 8g + sugar 20g + activated carbon 1g + banana meat 40g + potato mash 20g, pH=5.4.

[0039] Explant selection, sterilization, inoculation, transplantation and other operations are the same as in Example 1.

Embodiment 3

[0041] Control group 2, according to the Bletilla striata tissue culture rapid propagation medium reported in the literature, the sowing medium and the rooting medium adopt the medium formula of this patent, and the subculture medium adopts the medium formula reported in the literature, and the medium formula is as follows:

[0042] Each liter of seeding medium consists of the following components: 8g of agar + 30g of sugar + 80ml of coconut water + 0.05mg of 6-BA + 0.25mg of NAA, the rest is MS, pH=5.4.

[0043] Each liter of subculture medium consists of the following components: agar 6.5g + sucrose 30g + 6-BA 1.0mg + NAA 0.15mg, the rest is MS, pH=5.8.

[0044] Each liter of rooting colonization medium consists of the following components: Huabao No. 1 2g + Huabao No. 1g + agar 8g + sugar 20g + activated carbon 1g + banana meat 40g + potato mash 20g, pH=5.4.

[0045]Explant selection, sterilization, inoculation, transplantation and other operations are the same as in Exampl...

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Abstract

The invention discloses a culture medium for rapid propagation in tissue culture of bletilla striata, comprising a medium for bletilla striata seed sowing, a successive medium and a medium for rooting and planting. Compared to the prior art, the invention has the advantages of high germination, short culture time, big corm in acquired tissue culture plantlet, desirable effect of rooting and high transplanting survival rate, and has good market prospect.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a culture medium formula for tissue culture and rapid propagation of bletilla striata. Background technique [0002] Bletilla striata, (scientific name: Bletilla striata), also known as Lianjicao, Gangen, Baigi, Ruolan, Zhulan, Zilan, Zihui, Baili, is a perennial herb of the genus Bletilla striata in the family Orchidaceae, widely distributed in the Yangtze River Basin of my country Various provinces, mainly produced in Guizhou, Sichuan, Hunan, Hubei, Anhui, Henan, Zhejiang, Shaanxi and other places. Bletilla striata not only has high ornamental value, but also one of the important Chinese medicinal materials in my country. The tuber is used as medicine, which can astringe and stop bleeding, reduce swelling and promote muscle growth. It is widely used in the treatment of pulmonary tuberculosis, bronchiectasis, hemoptysis, and gastric ulcer bleeding. [0003] For a long time, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 江胜德郭兰付习杨兰兰续文华
Owner 虹越花卉股份有限公司
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