Application of thiadiazole compounds in inhibiting algae growth and enzyme activity and algicide for inhibiting algae growth

A technology of thiadiazoles and compounds, which is applied in the field of algicides that inhibit algae growth, can solve the problems of high toxicity, enhanced algae resistance, and poor selectivity of algicides, and achieve low toxicity, high inhibition rate, high efficiency and The effect of significant algicidal effect

Active Publication Date: 2017-04-05
HUAZHONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects of poor selectivity, high toxicity and increasing algae resistance of the prior art algicides, and provide a kind of algicide for inhibiting algae growth and thiadiazole compounds in inhibiting algae growth Applications

Method used

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  • Application of thiadiazole compounds in inhibiting algae growth and enzyme activity and algicide for inhibiting algae growth
  • Application of thiadiazole compounds in inhibiting algae growth and enzyme activity and algicide for inhibiting algae growth
  • Application of thiadiazole compounds in inhibiting algae growth and enzyme activity and algicide for inhibiting algae growth

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Experimental program
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Effect test

Embodiment 1

[0061] (1) Take the compounds in Table 1 of the present invention respectively, and dissolve the thiadiazole compounds with DMSO to form thiadiazole compounds with different concentrations such as 0, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30mM, etc. solution.

[0062] (2) The reaction buffer system for measuring fructose-1,6- / sedoheptulose-1,7-bisphosphatase enzyme activity contains 50mM Tris-HCl (pH 8.3), 10mM dithiothreitol ( DTT), 15mM MgCl 2 , 0.04 μg of fructose-1,6- / sedoheptulose-1,7-bisphosphatase. Add 100 μL of the above reaction buffer to each well of the 96-well plate, and then add 0.5 μL of the thiadiazole compound solutions with the above concentrations of 0, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, and 30 mM . Each concentration of compound was used in 3 groups in parallel, and a compound-free blank control (DMSO added, without reaction buffer) and compound background control (compound system prepared by dissolving in DMSO, without reaction buffer) were set at the same time. ...

Embodiment 2

[0068] Synechocystis PCC 6803 is the most important model algae among cyanobacteria. In this example, Synechocystis sp. PCC6803 was selected as the in vivo experiment object to test the effect of the thiadiazole compounds of the present invention on inhibiting the growth of algae.

[0069] The same amount of Synechocystis PCC 6803 was cultured in BG-11(+N) liquid medium containing different concentrations of compounds, and cultured in an artificial climate incubator. The temperature of the incubator is controlled at 28°C±1°C, the humidity is 60%, the light intensity is 6000 lx, 12h (light): 12h (dark), vibration mode, and manual shaking 3 times a day.

[0070] The specific operation is as follows:

[0071] Pre-cultivate a batch of Synechocystis PCC 6803, after 7 days of growth to the logarithmic phase, measure its OD 680 , ready for inoculation;

[0072] Dilute the algae liquid in the logarithmic phase with fresh BG-11(+N) medium to obtain the inoculum liquid, and control t...

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Abstract

The invention relates to the field of pesticide, and discloses an application of a thiadiazole compound shown in formula (I) in inhibiting the growth of algae and activity of algae fructose-1, 6- / sedoheptulose-1,7-biphosphatase, and discloses algicide for inhibiting the growth of algae. An active component of the algicide is at least one of thiadiazole compounds shown in the formula (I). According to the total weight of the algicide, the content of the active component is 0.1 to 100 percent by weight. The thiadiazole compound has advantages of high inhibition rate and small toxicity when in inhibiting the growth of algae. (See the specifications).

Description

technical field [0001] The invention relates to the field of pesticides, in particular to the application of thiadiazole compounds in inhibiting the growth of algae, and the application of thiadiazole compounds in inhibiting algae fructose-1,6- / sedoheptulose-1,7-diphosphate Applications in the activity of enzymes and algicides for inhibiting the growth of algae. Background technique [0002] In recent years, large-scale algae blooms have occurred continuously in various places. The algae damage directly affects domestic and industrial water in surrounding areas, seriously damages the ecological environment, brings huge economic losses to fishery production and tourism development, and also poses a threat to public health. Great hidden danger. [0003] The use of chemical algicides has the advantages of economy, high efficiency, convenience, and quick effect, and has always been an indispensable and important part of the comprehensive algae management system, especially duri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/824A01P13/00
Inventor 冯玲玲秦玉昌万坚赵晓晶韩强冯江涛李俊
Owner HUAZHONG NORMAL UNIV
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