Culture method of DC cell for enhancing CTL immune response
A cell culture, immune response technology, applied in the direction of blood/immune system cells, animal cells, vertebrate cells, etc., can solve the problems of ineffective activation of cytotoxic T cell proliferation and killing ability, long cycle, etc., to achieve effective in vivo. The effect of killing tumor, promoting proliferation and improving proliferation
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[0065] A DC cell culture method for enhancing CTL immune response, comprising the following steps:
[0066] 1), collect blood, prepare adherent mononuclear cells;
[0067] ①. Collect freshly collected peripheral blood or umbilical cord blood in a blood collection bag containing heparin sodium anticoagulant, shake it up quickly to avoid coagulation; when it needs to be transported, put the blood bag and biological ice bag together in a special incubator Keep the temperature at 4-25°C, and the temperature difference should not exceed ±5°C. Ships to the lab within 6 hours. During transportation, ensure that the blood is not irradiated by X-rays and kept away from radiation sources.
[0068] ②. Dilute and mix the blood with an equal volume of normal saline. According to the volume ratio of the diluted blood to the lymphocyte separation medium is 3:2, slowly superimpose the diluted blood on the lymphocyte separation medium in the centrifuge tube. Pay attention to keep the liquid...
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