A kit for detecting hpv16/18 based on digital PCR for accurate quantitative typing and its detection method
A quantitative typing and kit technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganism measurement/inspection, etc., can solve the problem of no typing kit for HPV16/18 detection, and achieve result interpretation Intuitive and reliable, good accuracy and reproducibility, simplified operation steps
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[0040] In order to better understand the content of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the following specific examples are only used to illustrate the present invention, rather than limit the present invention.
[0041] 1. A kit for detecting HPV16 / 18 based on digital PCR accurate quantitative typing, including DNA extraction solution, digital PCR reaction buffer A, digital PCR reaction buffer B, HPV16 virus gene positive quality control, HPV18 virus gene positive quality control, negative quality control and internal standard solution;
[0042] The DNA extract comprises 50 mM Tris-HCl with pH 8.3, 0.5 M NaCl, 5 mM EDTA, CTAB with a mass concentration of 1.5%, and DTT with 2 mM;
[0043] The digital PCR reaction buffer A includes primers and probes of the HPV16 type and the primers and probes of the HPV18 type; the digital PCR reaction...
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