Detection system and kit for detecting PDGFRA gene mutation

A system and genetic technology, applied in the field of detection products for detecting PDGFRA gene mutations, can solve the problems that GIST patients cannot benefit from it, and achieve the effects of absolutely accurate quantitative analysis, improved sensitivity, good accuracy and reproducibility

Pending Publication Date: 2018-10-19
上海赛安生物医药科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, if the D842V, RD841-842KI or D1842-843IM mutation occurs in the exon 18 gene locus, GIST patients cannot benefit from the treatment with tyrosine kinase inhibitors

Method used

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  • Detection system and kit for detecting PDGFRA gene mutation
  • Detection system and kit for detecting PDGFRA gene mutation
  • Detection system and kit for detecting PDGFRA gene mutation

Examples

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Embodiment Construction

[0040] The present invention is specifically described below through the examples, it is necessary to point out that the following examples are only used to further illustrate the present invention, and can not be interpreted as limiting the protection scope of the present invention, those skilled in the art can according to the above-mentioned present invention Contents Some non-essential improvements and adjustments are made to the present invention. In the following examples, unless otherwise specified, all reagents used are of analytical grade, and all reagents used can be obtained from commercial sources. For the experimental methods that do not indicate the specific conditions in the text, usually follow the conditions described in the book "Molecular Cloning Experiment Guide" published by Science Press, edited by J. Sambrook et al. in 2002, or according to the manufacturer's suggestion conditions of. Unless otherwise defined, all professional and scientific terms used ...

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Abstract

The invention relates to a detection system and kit for detecting PDGFRA gene mutation, wherein the detection system comprises a digital PCR premixed solution and a primer probe mixed solution; the primer probe mixed solution comprises upstream and downstream primers A and a peptide nucleic acid probe A for detecting a twelfth exon V516D locus, and upstream and downstream primers B and a peptide nucleic acid probe B for detecting a 18th exon D842V locus; the nucleotide sequence of the peptide nucleic acid probe A is completely complementary to a PDGFRA gene V516D mutation locus, and the nucleotide sequence of the peptide nucleic acid probe B is completely complementary to a PDGFRA gene D842V mutation locus; a 5' end of the peptide nucleic acid probes is connected with a strong chelating group composed of four amino acids. The detection system and kit for detecting the PDGFRA gene mutation have high sensitivity and specificity and low sample demand, so the PCR quantitative detection canbe carried out quickly, conveniently and accurately.

Description

technical field [0001] The invention relates to a detection product for detecting PDGFRA gene mutation, which belongs to the field of biotechnology. Background technique [0002] PDGFRA is one of the platelet-derived growth factor receptors (Platelet-Derived Growth Factor Receptor), a member of the tyrosine protein kinase family, which can promote the chemotactic division and proliferation of cells, and plays an important role in the process of body growth and development, wound repair, etc. Its overactivation and abnormal expression can induce the formation of tumor angiogenesis, and directly or indirectly promote the proliferation and migration of tumor cells. The PDGFRA gene is located on chromosome 4q11-4q12. Mutations are common in gastrointestinal stromal tumors, glioblastomas, and malignant peripheral nerve sheath tumors. Among them, the PDGFRA gene mutation rate in GIST is about 5-10%, and mutations mainly occur in genes The mutation frequencies of the proximal memb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858C12Q1/6851
CPCC12Q1/6851C12Q1/6858C12Q2537/143C12Q2563/159C12Q2525/107
Inventor 王明赵国玲洪专
Owner 上海赛安生物医药科技股份有限公司
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