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Sandy soil microbe solidification method and apparatus

A technology of curing device and curing method, applied in soil protection, construction, infrastructure engineering and other directions, can solve problems such as environmental hazards, and achieve broad application prospects, good social and economic benefits.

Active Publication Date: 2015-11-11
SOUTHEAST UNIV
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Problems solved by technology

[0004] Technical problem: The present invention aims at the environmental hazards existing in the domestic traditional solidifying agent to strengthen the soil, and proposes a microbial solidification method and device for sandy soil, which solves the environment-friendly problem of traditional calcium-based solidifying agent improving the soil, saving It saves social resources, has good economic benefits and engineering application prospects

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  • Sandy soil microbe solidification method and apparatus

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Embodiment Construction

[0018] A method and device for microbial solidification of sand, the steps of which are:

[0019] The first step is to prepare a high-concentration bacterial solution: inoculate Sporosarcina pasteurii (Sporosarcinapasteurii) into the ammonium salt-yeast-based culture solution, which contains 0.15mol / L tricarboxymethylaminomethane buffer solution, 10g / L(NH 4 ) 2 SO 4 , 20g / L yeast extract and 20g / L sugar, adjust the pH value of the culture medium to 8.0-9.0, place in a constant temperature vibration water bath at 30°C for 30-40h, and the vibration speed is 200 rpm, and wait for the light of the strain Density reaches OD 600 , take it out and centrifuge at a centrifugal acceleration of 4000g for 20min, and then centrifuge to obtain a concentration of 0.8~1.2×10 7 cells / mL high-concentration bacterial liquid;

[0020] The second step is to prepare the cementing medium solution: the cementing medium solution includes urea-calcium chloride (CO(NH 2 ) 2 -CaCl 2 ), ammonium ...

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Abstract

The invention discloses a sandy soil microbe solidification method and apparatus. The solidification method comprises steps of first, preparing high concentration bacteria liquid, second, compounding cementation medium solution, third, injecting the high concentration bacteria liquid and cementation medium solution into sandy oil, and fourth, nursing the sandy soil and molding the same. In the high concentration bacteria liquid preparation step, Pasteur spore sarcina is transplanted into an ammonium salt-yeast base nutrient solution; the PH value of the nutrient solution is adjusted to be about 8.0 to 9.0; the nutrient solution is vibratingly water-bathed for 30 to 40 hours at the temperature of 30 DEG C; then high concentration bacteria liquid with concentration of 0.8 to 1.2x107cells / ml is centrifugally achieved; the cementation medium solution comprises urea-calcium chloride, ammonium chloride, sodium bicarbonate and ammonium salt-yeast base nutrient solution; in the third step, the sandy soil is manufactured into samples with various dry density; two ends of each sample are respectively provided with a plastic block; the external side of the sample is covered with latex film; 15mL high concentration bacteria liquid and 50 to 100mL cementation medium solution are successively injected into the sandy oil via a peristaltic pump; and the samples are placed and nursed in environment with temperature ranging from 22 to 20 DEG C and relative humidity of 95%. With action of microbe solidification, unconfined compression strength can be obviously improved for the sandy soil.

Description

technical field [0001] The invention relates to a novel curing agent formula and a test device for microorganism-reinforced sandy soil, belonging to a method for foundation reinforcement and building restoration in the field of geotechnical engineering foundation treatment. Background technique [0002] With the continuous development of my country's economic construction and urbanization process, the relative shortage of road traffic and infrastructure has gradually become a social problem that needs to be solved urgently. In engineering construction, some unfavorable soils that cannot be directly used in engineering construction are often encountered. There are two common treatment methods: one is to remove the unfavorable soil and replace it with soil with better engineering characteristics; Add curing agent to the soil to improve the engineering properties of the soil and make it meet the requirements of engineering construction. The former is labor-intensive and time-c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E02D3/12
Inventor 张涛蔡国军段伟宏
Owner SOUTHEAST UNIV
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