Methods for inducing 'Hort 16A' offspring superior plant variation by using EMS and AFLP detection

Abstract

The invention relates to methods for inducing 'Hort 16A' offspring superior plant variation by using EMS and AFLP detection, and relates to a kiwifruit breeding and identification method. According to the invention, the method for directly adding the EMS into a culture medium is adopted to induce kiwifruit, and the induction effect of the EMS with the concentration of 0.08 mg / L to an embryonic callus is relatively remarkable. In the AFLP, the influence of the enzyme use quantity, enzyme digestion time and enzyme activity required by PCR amplification to the enzyme digestion is researched; the influence of the enzyme activity and energy to primer connection is analyzed; appropriate primer combinations are screened out in a selective amplification system to be used as selective amplification primers after DNA pre-amplification, so that the selective amplification of polymorphic DNA is facilitated, and the reliability for reflecting the fragment length polymorphism of the polymorphic DNA is improved; therefore, the purpose of detecting the small difference of DNA levels of mutants generated through induction by one-time detection can be achieved.

Description

technical field [0001] The invention belongs to plant breeding and identification methods, in particular to kiwifruit breeding and identification methods. Background technique [0002] Kiwifruit (kiwifruit) belongs to Actinidiacea (Actinidiacea, Actinidia spp. ), is a perennial deciduous vine, dioecious. Kiwifruit is a species of wild fruit trees artificially domesticated and cultivated. By artificially inducing mutations and mutations of kiwifruit, varieties with new flavors or new traits that do not exist in nature or that are difficult to obtain by conventional methods can be produced. However, there are few reports on the research on the variation breeding of kiwifruit treated with EMS. Document 1 (Long Zili, Study on in vitro regeneration and mutation induction of kiwifruit[D] . : Zhejiang University, 2008.) used EMS to treat kiwifruit callus to induce its adventitious shoot variation; Document 2 (Zhou Liming, Wang Fei, Wang Jia, EMS mutagenesis treatment directed sc...

AI Technical Summary

Problems solved by technology

But both use EMS alone to soak the callus for a long time, and then transfer it to the medium to induce kiwifruit, which takes a long time

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