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Rapid differentiation medium for Alhagi sparsifolia tissue culture, and preparation method thereof

A technology of differentiation medium and tissue culture, applied in the fields of botanical equipment and methods, horticultural methods, horticultural tools/equipment, etc., can solve problems such as inability to rapidly promote stem and leaf differentiation, underdeveloped camel thorn root system, and susceptible bacteria, etc. To achieve the effect of shortening the sprouting time, shortening the sprouting time, and reducing pollution

Active Publication Date: 2015-11-18
NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of its special physiological characteristics, if the commonly used auxin indole acetic acid is added to the differentiation medium at a low concentration, it cannot quickly promote stem and leaf differentiation. The concentration of indoleacetic acid is too high, which inhibits the growth and development of the root system, resulting in the hypoplasia of the root system of the tissue cultured camel thorn, so it is necessary to solve the problem of rapid differentiation of the tissue cultured camel thorn

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] The 1 / 2MS culture medium composition and consumption thereof (consumption refers to the consumption in 1 liter of 1 / 2MS culture medium) that the present invention selects for use are as follows table 1:

[0020]

[0021] Then add the following components to the 1 / 2MS medium: special curing agent, brown sugar, 6-benzylaminoadenine, 4-chloro-IAA, tetracycline, activated carbon, plant extracts, papaya juice, prepared in the differentiation medium The concentrations in the water are: special curing agent 15-18g / L, brown sugar 20-25g / L, tetracycline 25-45mg / L, 6-benzylaminoadenine 2.6-2.8mg / L, 4-chloro-IAA0.4- 0.5mg / L, plant extract 60-90mg / L, papaya juice 60-80g / L, activated carbon 3-4g / L. The concentration of each component is preferably 16g / L of special curing agent, 22g / L of brown sugar, 35mg / L of tetracycline, 2.7mg / L of 6-benzylaminoadenine, 0.45mg / L of 4-chloro-IAA, and 75mg / L of plant extract L, papaya juice 70g / L, activated carbon 4g / L. The preparation proces...

experiment example

[0026] According to the experimental ideas in the following table 2, the effect of the present invention is verified, all based on MS medium, in which one or more substances in the following table are selectively added according to the data requirements of the examples, wherein the concentration of IAA added in each group Equivalent to the 4-chloro-IAA mentioned in the examples.

[0027] Table 2:

[0028] group number Plant extracts 4-Chloro-IAA IAA 1 none none none 2 Have none none 3 none Have none 4 none none Have 5 Have Have none 6 Have none Have 7 none Have Have 8 Have Have Have

[0029] Fifty explants were selected from each group to carry out tissue culture experiments to count the germination time data, and the following table 3 was prepared with the average number:

[0030] Table 3: (unit: day)

[0031] group number 1 2 3 4 5 6 7 8 time 21.6 16.3...

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Abstract

The invention belongs to the technical field of tissue culture, and concretely relates to a rapid differentiation medium for Alhagi sparsifolia tissue culture. The differentiation medium is prepared through adding a specially prepared solidifying agent, brown sugar, 6-benzylamino adenine, 4-chloro-IAA, tetracycline, active carbon, plant extract and a pawpaw juice to a 1 / 2MS medium. The solidifying agent prepared through using sodium alginate and purple sweet potato extract is specially selected according to the special growth and physiology characteristics of Alhagi sparsifolia, and tetracycline is added to the medium to reduce pollution in the tissue culture process and promote germination of axillary buds in order to shorten the germinating time. Extract extracted from unprepared root of Ficus virens cooperates with 4-chloro-IAA to obviously promote germination and growth of axillalry buds. The survival rate of the medium is 98-99%, the pollution rate is 1.7-2.1%, the browning degree of the medium is 12-16% lower than that of the MS medium, and the germinating time shortens by about 20-43%.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a rapid differentiation medium for camel thorn tissue culture and a preparation method thereof. Background technique [0002] Camel thorn (scientific name: Alhagisparsifolia Shap.) belongs to the family Fabaceae, deciduous herb, with thorns on the main branches, oblong leaves, pink flowers, blooming in June, the most prosperous in August, each flower can be open for more than 20 days, pods, raceme Inflorescences, roots generally up to 20 m long. It is a naturally growing drought-tolerant plant that absorbs underground water and nutrients from the depths of deserts and Gobi. Because the stem of this plant has small, hard green leaves in the shape of thorns, it is called camel thorn. It is a herbaceous plant and a Gobi Camel grass is the only grass that camels can eat to survive in beaches and deserts, so it is also called camel grass, and it is mainly distri...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 徐伟明
Owner NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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