Vaginal secretion staining fluid, and preparation method and staining method thereof

A technique for vaginal secretions and staining solution, applied in the field of cell staining, which can solve the problems of long staining time, unfavorable large-scale clinical application, cumbersome staining solution operation steps, etc., to shorten the staining time, speed up the screening speed, and improve the staining efficiency Effect
CN105067412AActive Publication Date: 2015-11-18DIRUI MEDICAL TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DIRUI MEDICAL TECH CO LTD
Publication Date
2015-11-18

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Abstract

The invention discloses a vaginal secretion staining fluid, and a preparation method and a staining method thereof. According to the preparation method, a blue basic dyestuff and a red basic dyestuff are mixed so as to obtain the vaginal secretion staining fluid, so that the vaginal secretion staining fluid possesses staining effects of universal staining fluid, staining time can be shortened greatly, staining time is shortened from general 5-30min to 20s, staining efficiency is increased greatly, and screening speed of clinical samples is accelerated. And in addition, imagines obtained via staining with the vaginal secretion staining fluid are clear, and are convenient for identification; surface layer squamous epithelial cells, middle layer epithelial cells, bottom layer epithelial cells, clue cells, leukocytes, erythrocytes, monilia albicans, and trichomonad can be identified clearly; and the vaginal secretion staining fluid is suitable for large-scale sample clinical screening.
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Description

technical field

[0001] The invention relates to the technical field of cell staining, in particular to a vaginal secretion staining solution, a preparation method and a staining method thereof. Background technique

[0002] The chromatin in the nucleus is mainly deoxyribonucleic acid (DNA). In the DNA double helix structure, the phosphate groups on the two strands are facing outwards, negatively charged, and easily bonded with positively charged basic dyes by ionic bonds or hydrogen bonds. Bind, thereby attaching the corresponding color to the chromatin. The nucleus is the storage place of chromatin. If the chromatin is dense, the nucleus will be darkly stained; if the chromatin is loose, the nucleus will be lightly stained. The isoelectric point PI of the protein in the cytoplasm of the secretion is 3-6. When the pH>PI, the free carboxyl groups in the protein increase, which can be combined with the positively charged basic dye, so that the cytoplasm is attached to the ...

Claims

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