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A varroa destructor Anderson&Trueman saliva mixture and a preparation method and application thereof

A technology of Varroa dissaliva and its mixture, applied in the field of Varrodis saliva mixture and its preparation

Active Publication Date: 2015-11-25
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this uncapping and removal behavior is not specific to Varroa mites, but a general cleaning behavior, targeting all foreign substances or larvae endangered by microorganisms

Method used

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  • A varroa destructor Anderson&Trueman saliva mixture and a preparation method and application thereof
  • A varroa destructor Anderson&Trueman saliva mixture and a preparation method and application thereof
  • A varroa destructor Anderson&Trueman saliva mixture and a preparation method and application thereof

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Embodiment 1

[0017] Experimental materials and methods

[0018] 1. Obtaining the saliva mixture of Varroa mite

[0019] Referring to the method of Richards et al. (2011), the specific steps are as follows:

[0020] (a) collect the Varroa dissimilar in the honeycomb of the Italian bee workers with a fine brush, wash 2 times with sterile water containing 0.01% (mass fraction) of methylparaben;

[0021] (b) Add a absorbent cotton ball (sterilized, 121°C, 1h) to the petri dish, and add 120uLTC-100 (insect tissue culture medium, Sigma-Aidrich, T3160) on top;

[0022] (c) 33 Varroa mites were placed in a petri dish, 33 μL of pilocarpine solution (50 mg / ml) was dropped on the Varroa mite, and the Varroa mite was gently brushed with a brush to make the pilocarpine solution evenly distributed.

[0023] (d) overnight at 25°C, remove mites, soak cotton balls in 120 μL of TC-100 containing 50 μM phenylthiocarbamide, 0.008% methylparaben and 200 μg / mL ampicillin;

[0024] (e) Centrifuge: 4° C., 1300...

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Abstract

The invention provides a varroa destructor Anderson&Trueman saliva mixture and a preparation method and application thereof. It is discovered for the first time that the saliva of varroa destructor Anderson&Trueman has a lethal effect on prepupae of workers of apis cerana, and through further purification analysis, a purified varroa destructor Anderson&Trueman saliva mixture still has a lethal effect on prepupae of workers of apis cerana. Therefore, both the saliva and the purified saliva mixture can be used for preparing bee prepupa killing preparations. The purified varroa destructor Anderson&Trueman saliva mixture contains active substances capable of killing prepupae of workers of apis cerana. The invention creates conditions for basic scientific research on the mechanism of varroa destructor Anderson&Trueman hazards to bees.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a mixture of Varroa dissaliva and its preparation method and application. Background technique: [0002] Beekeeping is a characteristic industry of our country and also a traditional industry of our country. At present, the types of honeybees raised in my country are mainly Italian honeybee (Apismellifera) and Chinese honeybee (Apiscerana). my country is the world's largest producer and exporter of bee products as it is rich in nectar plants and a wide variety of bee products. The number of existing bee colonies reaches 7 million groups, accounting for about 12% of the total number of bees in the world. The annual output of honey is 280,000 tons, accounting for 20% of the world's honey production. The annual output of royal jelly is about 3,000 tons, accounting for more than 95% of the world's trade volume. In addition, the annual output of bee pollen is 3,500-4,000 to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 张祎韩日畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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