Unlock instant, AI-driven research and patent intelligence for your innovation.

A marine bacterium and its exopolysaccharide

A technology for marine bacteria and extracellular polysaccharides, applied in the field of extracellular polysaccharides, can solve problems such as limited bacterial polysaccharides

Active Publication Date: 2018-07-31
XIAMEN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bacterial polysaccharides currently used in practical production are very limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A marine bacterium and its exopolysaccharide
  • A marine bacterium and its exopolysaccharide
  • A marine bacterium and its exopolysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The acquisition of embodiment 1 bacterial strain

[0028] Bacterial strain of the present invention obtains by following method screening: seawater is collected in August, 2012, longitude 115 ° E, latitude 18 ° N, depth of water 75m. Pipette 100 μL of the collected seawater onto the 2216E plate. 2216E medium: peptone 5.0g, yeast extract 1.0g, iron phosphate 0.01g, agar 20.0g, artificial seawater 1000ml, adjust ph to 7.6. Place at room temperature for incubation. After 3 days of culture, colonies with different shapes and sizes were randomly picked from the plate for purification and culture. When purifying, use an inoculation loop to pick colonies, and streak on the surface of the plate to observe whether the color, size, and shape of the strains on the plate are the same. Pick different colonies and streak until a colony of a single form is isolated. The polysaccharide-producing strain was detected by the phenol-sulfuric acid method to detect whether the culture sup...

Embodiment 2

[0032] The extraction of embodiment 2 polysaccharides

[0033] Determination of polysaccharide molecular weight

[0034] The molecular weight of polysaccharides was determined by high performance liquid chromatography. Polysaccharides were analyzed using a size exclusion column. Chromatographic column The column is made of TSK Gel GS3000SW XL Gel chromatography column (7.8mmID×30cm) and TSK GelG2000SW XL Gel chromatography columns (7.8mm ID×30cm) are connected in series, and the detector is a refraction detector. The mobile phase was 0.1 M ammonium acetate containing 0.2‰ sodium azide. The temperature of the column was set at 30° C., and the flow rate was set at 0.6 mL / min. Standard solutions include dextran (511, 167, 67, 40, 10, 5, 1 kDa) and maltotetraose (666.6 Da) with different molecular weights. Polysaccharides with different molecular weights have different retention times on size exclusion chromatography columns (see Table 1). According to the correlation betwe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
diameteraaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

A strain of marine bacteria and the exopolysaccharide it produces relate to a strain of marine bacteria. Said one strain of marine bacteria Alteromonase marina P2-B12, the registration number of the preservation center: CGMCC No.11180. A method for preparing exopolysaccharides from a marine bacterium Alteromonase marina P2‑B12: 1) strain cultivation; 2) extraction and desalination of polysaccharides; 3) separation and purification. The molecular weight of the prepared marine bacterial exopolysaccharide is greater than 167kDa, and the marine bacterial exopolysaccharide is composed of rhamnose, mannose and galacturonic acid with α-1,3 and α-1,4 bonds to form a structural unit ([- 3]‑α‑Rhap‑(1→3)‑α‑Manp‑(1→4)‑α‑GalAp‑(1→)).

Description

technical field [0001] The invention relates to a strain of marine bacteria, in particular to a strain of marine bacteria and the exopolysaccharide produced therefrom. Background technique [0002] Bacteria generally synthesize and secrete exopolysaccharides. Exopolysaccharides are secondary metabolites of bacteria, which are soluble polysaccharide complexes that are secreted outside the cell wall during bacterial growth and separated from the bacteria. Due to their unique living environment, the exopolysaccharides secreted by marine bacteria often have special structures. Therefore, the exopolysaccharides produced by marine bacteria have great potential application value in the field of biotechnology. Some exopolysaccharide-producing bacteria have been isolated from different marine environments, for example, abundant exopolysaccharide-producing bacteria have been found from deep-sea hydrothermal vents, marine volcanoes and hydrothermal areas, and shallow seabed hot spring...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04C08B37/00C12R1/01
Inventor 张子莲焦念志蔡阮鸿
Owner XIAMEN UNIV