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Application of poly(sebacoyl diglyceride) in preparation of retinal nerve cell carrier

A technology of sebacoyl diglyceride and sebacoyl glycerol, which is applied in the field of cell carrier and its preparation, can solve the problem that the disease cannot be fundamentally treated, and achieve excellent neural differentiation-promoting function, low apoptosis factor, good The effect of biocompatibility

Active Publication Date: 2015-12-09
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, these treatments can only delay the deterioration of the disease, but cannot fundamentally treat the disease.

Method used

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  • Application of poly(sebacoyl diglyceride) in preparation of retinal nerve cell carrier
  • Application of poly(sebacoyl diglyceride) in preparation of retinal nerve cell carrier
  • Application of poly(sebacoyl diglyceride) in preparation of retinal nerve cell carrier

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0017] Synthesis of PSeDs.

[0018] Diglycidyl sebacate monomer, equimolar amount of sebacic acid and catalytic amount of tetrabutylammonium bromide (TCI, >99.0%) were dissolved in DMF, and heated in a nitrogen-protected dry reaction tube ( 150°C) for 24 hours. Then the reaction solution was purified by ether precipitation three times, and then the precipitate was vacuum-dried to obtain PSeD.

[0019] Through the isolation and culture of RPCs, the effect of PSeD / PLGA on the proliferation and differentiation of RPCs, immunocytochemistry, Western blot analysis, total RNA isolation and quality control, reverse transcription and quantitative polymerase chain reaction (qPCR), cytotoxicity analysis, trauma Healing and statistical analysis to characterize the effect of PSeD on the behavior of RPCs.

[0020] NC in the examples means no material blank test group.

[0021] (1) Isolation and culture of RPCs

[0022] The animals used in the present invention are all in accordance with...

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Abstract

The invention relates to an application of poly(sebacoyl diglyceride) in preparation of a retinal nerve cell carrier; retinal progenitor cells RPCs are proliferated, differentiated and migrated on the retinal nerve cell carrier, wherein a component of the retinal nerve cell carrier comprises poly(sebacoyl diglyceride) PSeD. The retinal nerve cell delivery carrier has the advantages of good biological compatibility, low cell inflammation expression, low cell apoptosis factor, and excellent neural differentiation promoting function.

Description

technical field [0001] The invention belongs to the field of cell carriers and preparation methods thereof, in particular to the application of poly(sebacyl diglyceride) in the preparation of retinal nerve cell carriers. Background technique [0002] Age-related macular degeneration (AMD) and retinitis pigmentosa (RP) are two major contributors to the degeneration of optic neurons leading to visual impairment. Newly developed treatments can rebuild the damaged retinal neurons. As a result, a variety of therapeutic methods have been developed, such as gene therapy, growth factor therapy and small molecule drug therapy. However, these treatment methods can only delay the progression of the disease, but cannot fundamentally treat the disease. In recent years, data have shown that transplantation of retinal neurons cultured in vitro to replace diseased tissue in vivo has attracted more and more attention. But finding a suitable cell culture substrate as a delivery vehicle for...

Claims

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Application Information

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IPC IPC(8): C12N5/071C08G63/668C08G63/78
Inventor 游正伟陈硕王少飞谷平倪妮
Owner DONGHUA UNIV
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