Compositions including a botanical extract and methods of using the same in skin whitening and skin lightening applications

A chrysanthemum flower extract, a technology for skin whitening, applied to a composition containing the chrysanthemum flower extract and its use in the fields of skin whitening and skin brightening applications, can solve problems such as skin irritation or acute dermatitis

Inactive Publication Date: 2015-12-16
ACCESS BUSINESS GRP INT LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] One concern with synthetic skin lighteners, such as hydroquinone or kojic acid, is that they may cause skin irritation or acute dermatitis

Method used

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  • Compositions including a botanical extract and methods of using the same in skin whitening and skin lightening applications
  • Compositions including a botanical extract and methods of using the same in skin whitening and skin lightening applications
  • Compositions including a botanical extract and methods of using the same in skin whitening and skin lightening applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1: Identification of Luteolin-7-Glucoside as an "Active" Marker for Skin Lightening Activity

[0080] In order to determine the active ingredients of the chrysanthemum extract, the following tests were performed.

[0081] Materials and Instruments:

[0082] All solvents were HPLC grade and purchased from Fisher Scientific. HPLC separations were achieved using an Agilent Technologies HP1100 System equipped with a Photodiode-array detector and Chemstation software. The chromatographic column was purchased from Agilent, ZorbaxSB-C185u4.6x250mm, product model: 880975-902

[0083] Standard compound:

[0084] Luteolin-7-O-glucoside, Chromadex product number: ASB-00012500-010 (CAS#:5371-11-5)

[0085] Sample Preparation:

[0086] Weigh about 400mg of chrysanthemum dry powder sample (approximately 0.1mg) and put it in a 50mL volumetric flask. Add approximately 40 mL of 80 / 20 methanol to DI water (diluent) and shake well to dissolve. The solution was placed in a s...

Embodiment 2

[0097] Example 2: Chrysanthemum extract standardized to about 1.0% luteolin-7-glucoside exhibits dose-dependent reduction in melanin production

[0098] In order to determine whether luteolin-7-glucoside present in chrysanthemum affects melanin production, the following experiments were performed.

[0099] A standardized chrysanthemum extract containing 20% ​​luteolin-7-glucoside was tested.

[0100] Amounts of 25 μg and 12.5 μg of chrysanthemum extract were dissolved in DMSO and added to melanoma cells at 5×10 4 cells at a density freshly seeded on a 24-well plate. The cytosolic pH of Melan-a melanocytes in this assay is about 5-7. Each dose of chrysanthemum extract was added to the cells in triplicate. The final concentration of DMSO in the medium was kept at 0.25%. Forty-eight hours after the first set of treatments, the cells were treated again with chrysanthemum extract and fed with fresh medium. Two days later, melanin was extracted and normalized by protein content...

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Abstract

Compositions for topical use and having a melanin synthesis-inhibiting activity that include an amount of a botanical extract of at least one of Aspalathus Linearis, Matricaria, Saxifraga, Astragalus, Taraxacum officinale / mongolicum, Ferula varia / foetida, Carthamus tinctorius, Sophora flavescentis, or Chrysanthemum morifolium effective to reduce melanin synthesis are described. Methods for inhibiting melanin synthesis and for skin whitening are also described.

Description

[0001] related application [0002] This patent document claims the benefit of the filing dates of U.S. Provisional Patent Applications 61 / 988,576, filed May 5, 2014, and 62 / 103,202, filed January 14, 2015, pursuant to 35 U.S.C. § 119(e), 61 / 988,576 and 62 / 103,202 are incorporated into this application by reference. technical field [0003] The present application describes compositions comprising plant extracts and their use in methods of skin whitening or lightening and methods of inhibiting melanin synthesis in the skin. Background technique [0004] Skin tone is primarily determined by the amount of melanin in the skin. Melanin is the dark brown pigment present in the skin. Due to the dark color of this pigment, lower amounts of melanin result in lighter skin tones and higher amounts result in darker skin tones. Melanin is formed by the oxidation of tyrosine to dihydroxyphenylalanine in melanocytes. This reaction is catalyzed by the enzyme tyrosinase. [0005] Exce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/287A61P17/00
CPCA61K8/602A61Q19/02A61K2800/782A61K36/185A61K36/23A61K36/28A61K36/286A61K36/287A61K36/288A61K36/48A61K36/481A61K36/489A61K8/9789A61P17/00A61K2300/00A61K2800/70
Inventor J.拉纳G.迪瓦卡X.沈J.斯科尔坦R.米勒
Owner ACCESS BUSINESS GRP INT LLC
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