Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A low-injury method for primary culture of colorectal cancer cells

A primary culture, colorectal technology, applied in the field of cell culture, can solve the problems of uneven cell digestion, affect cell activity, damage cell membrane, etc., achieve the effect of inhibiting bacterial reproduction, avoiding cell contamination, and improving the adherence rate

Inactive Publication Date: 2019-04-05
THE FIRST HOSPITAL OF HEBEI MEDICAL UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The traditional enzymatic digestion method has the following defects: due to the digestion time (one sample can only select the same processing time, for example, the digestion time is set according to the standard of digesting a slightly larger tissue block into a single cell, which will inevitably result in smaller tissue Overdigestion of lumps and free cells), will cause uneven digestion of cells or even excessive digestion, damage cell membranes, destroy cells, affect cell activity, or incomplete digestion is not conducive to high-throughput screening experiments; there are many types of enzymes, in digestion The type and concentration of enzymes are variable when selecting enzymes, and different tissues have different requirements for enzymes, which requires complex conditions to explore

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A low-injury method for primary culture of colorectal cancer cells
  • A low-injury method for primary culture of colorectal cancer cells
  • A low-injury method for primary culture of colorectal cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 A method for primary culture of colorectal cancer cells with low damage, which comprises the following steps:

[0052] (1) Tissue pretreatment: select colorectal cancer tissue, rinse with sterile saline for more than 30 seconds, soak in serum-free RPMI-1640 medium containing 4 high-concentration antibiotics and interleukin-6 inhibitors; after 5-10 minutes, replace Soak in fresh serum-free RPMI-1640 medium containing high concentrations of antibiotics for 5-10 minutes; during the soaking process, shake from time to time, upside down, 6 times / min, to better contact and clean each other; interleukin 6 inhibitors in the soaking solution Siltuximab was selected, and the interleukin-6 inhibitor was produced by Johnson & Johnson, with a concentration of 11 μg / mL;

[0053] This step can neutralize the interleukin 6 in the necrotic tissue and reduce the cell damage mediated by it. At the same time, this operation also lowers the standard of tissue sampling; the necr...

Embodiment 2

[0073] The difference between Example 2 and Example 1 is that the concentration of the interleukin-6 inhibitor Siltuximab is 1 μg / mL during the pretreatment of the tissue in step (1); 3 .

Embodiment 3

[0075] The difference between Example 3 and Example 1 is that the concentration of the interleukin-6 inhibitor Siltuximab is 15 μg / mL during the pretreatment of the tissue in step (1); 3 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a low-injury primary culture method for colorectal cancer cells. The low-injury primary culture method comprises the following steps: (1) cell pretreating: treating colorectal cancer tissues with antibiotics; (2) dicing; (3) culturing: putting the diced tissue blocks into a culture medium containing the antibiotics for primary culture and normal culture till cell attachment is realized. The primary culture method has the advantages that the injury to the cells is low; the survival rate of the cells is high; the operation is simple; the culture is easy.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a low-damage primary culture method for colorectal cancer cells. Background technique [0002] Colorectal cancer is a common malignant tumor of the digestive system. In recent years, the incidence and mortality of colorectal cancer in my country have been increasing year by year. Most colorectal cancer patients are in the middle and advanced stages when they go to the doctor, and the treatment costs are high, the effect is poor, and the long-term survival rate is low. At the same time, it brings a heavy burden to patients, families and society. Colorectal cancer has obvious precancerous and early cancer lesions, and the time lasts for more than ten years. This provides opportunities for early detection and early treatment of colorectal cancer and precancerous lesions. The determination of tumor markers plays an important role in tumor diagnosis, curative effec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09
Inventor 赵增仁翟从劼张峰姜霞唐宇杰
Owner THE FIRST HOSPITAL OF HEBEI MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products