A low-injury method for primary culture of colorectal cancer cells
A primary culture, colorectal technology, applied in the field of cell culture, can solve the problems of uneven cell digestion, affect cell activity, damage cell membrane, etc., achieve the effect of inhibiting bacterial reproduction, avoiding cell contamination, and improving the adherence rate
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Embodiment 1
[0051] Embodiment 1 A method for primary culture of colorectal cancer cells with low damage, which comprises the following steps:
[0052] (1) Tissue pretreatment: select colorectal cancer tissue, rinse with sterile saline for more than 30 seconds, soak in serum-free RPMI-1640 medium containing 4 high-concentration antibiotics and interleukin-6 inhibitors; after 5-10 minutes, replace Soak in fresh serum-free RPMI-1640 medium containing high concentrations of antibiotics for 5-10 minutes; during the soaking process, shake from time to time, upside down, 6 times / min, to better contact and clean each other; interleukin 6 inhibitors in the soaking solution Siltuximab was selected, and the interleukin-6 inhibitor was produced by Johnson & Johnson, with a concentration of 11 μg / mL;
[0053] This step can neutralize the interleukin 6 in the necrotic tissue and reduce the cell damage mediated by it. At the same time, this operation also lowers the standard of tissue sampling; the necr...
Embodiment 2
[0073] The difference between Example 2 and Example 1 is that the concentration of the interleukin-6 inhibitor Siltuximab is 1 μg / mL during the pretreatment of the tissue in step (1); 3 .
Embodiment 3
[0075] The difference between Example 3 and Example 1 is that the concentration of the interleukin-6 inhibitor Siltuximab is 15 μg / mL during the pretreatment of the tissue in step (1); 3 .
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