Preparing technology for active nano-selenium brain peptide

A preparation process, nano-selenium technology, which is applied in the field of preparation of active nano-selenium brain peptide, can solve the problems of danger, no patent report, and large consumption of organic solvents, so as to ensure stability and biological activity, avoid environmental pollution, and degrease good effect

Inactive Publication Date: 2015-12-16
陈石良
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technical solutions only prepare single-functional animal brain protein hydrolyzate or a certain form of elemental nano-selenium, and organically fuse nano-selenium with cerebroprotein or brain peptide to prepare more functionally active nano-selenium cerebroprotein or brain peptide No patent report yet
[0005] In addition, in the existing animal cerebroprotein or brain peptide extraction process, organic solvents (such a...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparing technology for active nano-selenium brain peptide
  • Preparing technology for active nano-selenium brain peptide
  • Preparing technology for active nano-selenium brain peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Take 200kg of fresh and healthy pig brain, remove mucous membranes, remove blood vessels, wash, add deionized water twice the weight of pig brain, and homogenize with a homogenizer to obtain brain plasma; (2) Brain plasma The temperature of the solution was raised to 35°C, the pH value was adjusted to 9.0, 0.1% lipase of the total weight of the brain plasma was added, stirred for 5 minutes, the stirring was stopped, and after 40 minutes of heat preservation for enzymolysis, the temperature was raised to 95°C to inactivate the enzyme for 20 minutes, and cooled to room temperature Finally, centrifuge at 4000rmp for 15 minutes to collect the precipitate, add 2 times the weight of the precipitate to the deionized water and stir evenly to obtain the defatted cerebroprotein solution; (3) raise the temperature of the defatted cerebroprotein solution to 50°C, add the defatted cerebroprotein solution 1.2% of the total weight of the compound enzyme, adjust the pH value to 8.0,...

Embodiment 2

[0024](1) Take 200kg of tested and healthy frozen fresh pig brain, remove mucous membranes, remove blood vessels, wash after thawing, add deionized water twice the weight of pig brain, and homogenize with a homogenizer to obtain brain plasma; (2) ) Raise the temperature of the brain plasma to 40°C, adjust the pH value to 9.5, add 0.05% lipase of the total weight of the brain plasma, stir for 5 minutes, stop stirring, keep warm for 50 minutes for enzymatic hydrolysis, then raise the temperature to 95°C to inactivate the enzyme for 20 minutes, After cooling to room temperature, centrifuge at 4000rmp for 15 minutes to collect the precipitate, add deionized water twice the weight of the precipitate to the precipitate and stir evenly to obtain the defatted cerebroprotein solution; (3) raise the temperature of the defatted cerebroprotein solution to 55°C, add 1.0% of the total weight of the cerebroprotein solution, adjust the pH value to 8.5, stir for 5 minutes, stop stirring, keep w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
The average diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a preparing technology for active nano-selenium brain peptide. The preparing technology includes the following steps that 1, pig brain tissues are homogenized, enzymolysis degreasing is carried out on the homogenized pig brain tissues through lipase, hydrolysis is carried out through complex enzymes, and ultra-filtration is carried out to obtain brain peptide liquid, wherein the quantity of the added lipase is 0.05% to 0.15% of the weight of brain liquid, and the quantity of the added complex enzymes is 0.2% to 2% of the weight of degreased brain protein liquid; 2, a sodium selenite solution and an ascorbic acid solution are added into the brain peptide liquid, stirring reacting is carried out for 1 hour to 2 hours at the temperature of 40 DEG C to 55 DEG C, and reaction liquid is obtained; 3, spraying drying is carried out on the reaction liquid, and the finished product is obtained. According to the preparing technology, brain protein hydrolysate is degreased in the enzymolysis mode, the degreasing effect is good, environment pollution is avoided, and the biological activity of small molecule peptide and the safety of the product are guaranteed; meanwhile, the small molecule brain peptide obtained after enzymolysis serves as a medium template to adsorb and stabilize nano-selenium, the active brain peptide is combined with the nano-selenium, the physiological functions of the brain peptide and the physiological functions of the nano-selenium are promoted and synergistically interact with each other, and the obtained product has the better treatment effect on various diseases such as insomnia, cephalalgia and hypomnesis.

Description

technical field [0001] The invention relates to a preparation method of brain protein powder, in particular to a preparation process of active nano selenium brain peptide extracted from pig brain tissue. Background technique [0002] Mammalian brain tissue is relatively compatible with human brain tissue, and various proteins, peptides, nerve growth factors, phospholipids, mucopolysaccharides, etc. contained in it can be used by human brain tissue. Using enzyme engineering technology, a large number of small molecular peptides, amino acids, coenzymes and other substances can be obtained from animal brain tissue, which is called brain protein hydrolyzate or brain peptide in the industry. According to research reports, more than 30 kinds of active peptides have been found in mammalian brain tissue. The functions of brain peptides are mainly manifested in two aspects: regulatory function and nutritional function. Regulatory functions include improving sleep and memory. Extend ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P21/06C07K1/34
Inventor 陈石良时亚文
Owner 陈石良
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap