Preparing technology for active nano-selenium brain peptide
A preparation process, nano-selenium technology, which is applied in the field of preparation of active nano-selenium brain peptide, can solve the problems of danger, no patent report, and large consumption of organic solvents, so as to ensure stability and biological activity, avoid environmental pollution, and degrease good effect
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Embodiment 1
[0022] (1) Take 200kg of fresh and healthy pig brain, remove mucous membranes, remove blood vessels, wash, add deionized water twice the weight of pig brain, and homogenize with a homogenizer to obtain brain plasma; (2) Brain plasma The temperature of the solution was raised to 35°C, the pH value was adjusted to 9.0, 0.1% lipase of the total weight of the brain plasma was added, stirred for 5 minutes, the stirring was stopped, and after 40 minutes of heat preservation for enzymolysis, the temperature was raised to 95°C to inactivate the enzyme for 20 minutes, and cooled to room temperature Finally, centrifuge at 4000rmp for 15 minutes to collect the precipitate, add 2 times the weight of the precipitate to the deionized water and stir evenly to obtain the defatted cerebroprotein solution; (3) raise the temperature of the defatted cerebroprotein solution to 50°C, add the defatted cerebroprotein solution 1.2% of the total weight of the compound enzyme, adjust the pH value to 8.0,...
Embodiment 2
[0024](1) Take 200kg of tested and healthy frozen fresh pig brain, remove mucous membranes, remove blood vessels, wash after thawing, add deionized water twice the weight of pig brain, and homogenize with a homogenizer to obtain brain plasma; (2) ) Raise the temperature of the brain plasma to 40°C, adjust the pH value to 9.5, add 0.05% lipase of the total weight of the brain plasma, stir for 5 minutes, stop stirring, keep warm for 50 minutes for enzymatic hydrolysis, then raise the temperature to 95°C to inactivate the enzyme for 20 minutes, After cooling to room temperature, centrifuge at 4000rmp for 15 minutes to collect the precipitate, add deionized water twice the weight of the precipitate to the precipitate and stir evenly to obtain the defatted cerebroprotein solution; (3) raise the temperature of the defatted cerebroprotein solution to 55°C, add 1.0% of the total weight of the cerebroprotein solution, adjust the pH value to 8.5, stir for 5 minutes, stop stirring, keep w...
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