Ochrobactrum and biological agent thereof, preparation method and application
The technology of Bacillus pallidum and biological preparation, which is applied in the field of microorganisms, can solve the problems of cumulative effect, pesticide residues, no product of thiophanate-methyl degrading agent, etc., and achieves the effects of convenient use and low cost.
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[0030] The preparation method of the biological preparation in which the palebacterium pallidus of the present invention is an active ingredient comprises the steps of test tube seed culture and liquid fermentation culture, specifically comprising:
[0031] A, test tube culture: the intermedium type paleobacterium ( Ochrobactrum intermedium ) TP206 was inoculated on the slant medium of the test tube, and cultured at a constant temperature of 28-31°C for 0.5-1.5 days to obtain the test-tube species;
[0032] B. Liquid fermentation culture: Inoculate the test tube seed into the liquid medium, and culture it on a shaking table at a temperature of 28-31°C for 1-3 days to obtain a biological preparation in which the active ingredient is the target object, Paleobacterium pallidum.
[0033] The composition of the test tube slant medium described in step A is: glucose 1~3g, beef extract 0.5~1.5g, sodium chloride 0.4~0.7g, MgSO 4 ·7H 2 O0.1-0.3g, agar 1.5-2g, the rest is water, pH val...
Embodiment 1
[0057] Paleobacterium intermedius ( Ochrobactrum intermedium ) The TP206 strain was inoculated on the agar slant of the test tube, and the medium formula was: glucose 1g, beef extract 1.0g, sodium chloride 0.5g, MgSO 4 ·7H 2 O 0.2g, agar 1.5g, distilled water 100ml, pH 6.5; 30 ℃ constant temperature culture for 1 day, to obtain test tube species.
[0058] Inoculate the cultured test tube species into 500ml Erlenmeyer flask liquid medium, fill each bottle with 200ml, ferment at 30°C for 2 days, and rotate at 180~200rpm. The medium formula is: glucose 1g, beef extract 0.2g, bean cake powder 1g, NaCl 0.3g, MgSO 4 ·7H 2 O0.2g, distilled water 100ml, pH value 6.5.
[0059] The culture time is 2 days, and the bacterial concentration is ≥25×10 per ml. 8 cfu / mL, a biological agent with the function of reducing thiophanate-methyl was obtained.
Embodiment 2
[0061] Same as Example 1, the difference lies in the formula of liquid medium, its formula is: glucose 2g, beef extract 0.25g, bean cake powder 1.2g, NaCl0.4g, MgSO 7H 2 O0.2g, distilled water 100ml, pH value 7. The concentration of bacteria is to reach the number of live bacteria per ml ≥ 34×10 8 cfu / mL.
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