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Weissella sinensis xhr1 and application thereof, kimchi containing Weissia sinensis xhr1

A technology of Weissella annulophaga and kimchi, which is applied in the field of kimchi containing Weissella anus sinus XHR1, can solve problems such as buried health risks, drug resistance of Candida albicans, and bacterial flora imbalance, and achieve improvement in microbiology. The effect of ecological level and wide range of consumer groups

Active Publication Date: 2019-02-19
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most antifungal drugs have broad-spectrum antibacterial properties, which will further cause the body's flora imbalance during treatment, bury health risks, and cause secondary effects on the body
Third, due to the commonness and recurrence of vulvovaginal candidiasis, repeated treatment with drugs can cause drug resistance of Candida albicans

Method used

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  • Weissella sinensis xhr1 and application thereof, kimchi containing Weissia sinensis xhr1
  • Weissella sinensis xhr1 and application thereof, kimchi containing Weissia sinensis xhr1
  • Weissella sinensis xhr1 and application thereof, kimchi containing Weissia sinensis xhr1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Weissella esophagus XHR1 is used for the fermentation of bamboo shoot shell vegetables

[0030] (1) Bacteria powder 1: Preparation of Weissella angustiella XHR1 bacteria powder

[0031]Inoculate 10L of Weissella sinusella XHR1 bacterial strain culture solution in the logarithmic growth phase into a fermenter of 1000L and carry out high-density cultivation, culture conditions: 1.90% (W / V, the same below) corn steep liquor, 2.00% glucose, 1.00 % whey powder, 1.20% yeast extract powder, 0.06% magnesium sulfate, 0.02% manganese sulfate, 0.20% dipotassium hydrogen phosphate, 0.80% sodium chloride, initial pH 5.8, fermentation temperature 30 ° C for 48 hours to obtain the cell density 5.25×10 9 CFU / mL fermentation broth; the fermentation broth is filtered through an 80-mesh filter, and the filtrate is added with 12% maltodextrin of the filtrate mass, 12% of the filtrate mass of porous starch and 0.1% of the filtrate mass of polyvinylpyrrolidone, and spray-dried...

Embodiment 2

[0036] Example 2: Weissella esophagus XHR1 for cabbage fermentation

[0037] (1) Bacteria powder 1: Preparation of Weissella angustiella XHR1 bacteria powder

[0038] Inoculate 10L of Weissella sinusella XHR1 bacterial strain culture solution in the logarithmic growth phase into a fermenter of 1000L and carry out high-density cultivation, culture conditions: 1.90% (W / V, the same below) corn steep liquor, 2.00% glucose, 1.00 % whey powder, 1.20% yeast extract powder, 0.06% magnesium sulfate, 0.02% manganese sulfate, 0.20% dipotassium hydrogen phosphate, 0.80% sodium chloride, initial pH 6.0, fermentation temperature 30 ° C for 48 hours to obtain the cell density 6.32×10 9 CFU / mL fermentation broth; the fermentation broth is filtered through an 80-mesh filter, and the filtrate is added with 12% maltodextrin of the filtrate mass, 12% of the filtrate mass of porous starch and 0.1% of the filtrate mass of polyvinylpyrrolidone, and spray-dried at low temperature Afterwards, add fi...

Embodiment 3

[0043] Example 3: Weissella esophagus XHR1 for carrot fermentation

[0044] (1) Bacteria powder 1: Preparation of Weissella angustiella XHR1 bacteria powder

[0045] Inoculate 10L of Weissella sinusella XHR1 bacterial strain culture solution in the logarithmic growth phase into a fermenter of 1000L and carry out high-density cultivation, culture conditions: 1.90% (W / V, the same below) corn steep liquor, 2.00% glucose, 1.00 % whey powder, 1.20% yeast extract powder, 0.06% magnesium sulfate, 0.02% manganese sulfate, 0.20% dipotassium hydrogen phosphate, 0.80% sodium chloride, initial pH 6.2, fermentation temperature 30 ° C for 48 hours to obtain the cell density 7.36×10 9 CFU / mL fermentation broth; the fermentation broth is filtered through an 80-mesh filter, and the filtrate is added with 12% maltodextrin of the filtrate mass, 12% of the filtrate mass of porous starch and 0.1% of the filtrate mass of polyvinylpyrrolidone, and spray-dried at low temperature Afterwards, add fil...

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Abstract

The invention provides a weissella cibaria XHR1 and applications thereof and a weissella cibaria XHR1 containing pickled vegetable. The preparation of the pickled vegetable is implemented through the steps of adding a culture solution of weissella cibaria XHR1 and bacterial powder of lactobacillus plantarum CICC 20764 into a pickled vegetable fermentation starting solution for pickling vegetables, so that the final concentration of weissella cibaria XHR1 is 10<6>-10<7> cfu / mL eventually, and then the final concentration of lactobacillus plantarum CICC 2076 is 10<5>-10<6> CFU / mL; and then, adding vegetables into the pickled vegetable fermentation starting solution, and carrying out vegetable pickling according to a traditional vegetable fermentation process, so that the pickled vegetable disclosed by the invention is obtained finally. According to the invention, prebiotic lactobacillus for inhibiting the growth of Candida albicans is applied to the fermentation of Sichuan pickled vegetables, so that the consumer group is wider, and the micro ecological level of alimentary canals of wide consumers can be improved, thereby inhibiting Candida albicans diseases from the perspective of public health.

Description

technical field [0001] The invention relates to the field of food technology, in particular to Weissella anus sinus XHR1 and application thereof, and pickles containing Weissella ansinus XHR1. Background technique [0002] Candida albicans (Candida albicans) is a symbiotic yeast in the human oral cavity, digestive tract and vagina. It is a common human pathogenic fungus, which can cause mucosal and systemic infections, especially in immunocompromised people. Candida is a normal parasite in the vagina, which plays an important role in maintaining the normal ecological balance in the vagina. Foreign statistics show that about 75% of women suffer from vulvovaginal candidiasis at least once in their lives, and 40%-50% of them suffer from 2 or more episodes. Candida albicans is also an important conditional pathogenic microorganism in the oral cavity, and it is the pathogenic bacteria of many oral diseases including children's "thrush". Drugs currently used to treat candidiasis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L19/20C12R1/01
Inventor 饶瑜李成龙何鹏晖杨建涛罗军蒋倩常伟车振明
Owner XIHUA UNIV
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