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Gene detecting primers of sickle cell anemia and composition of gene detecting primers

A gene detection and anemia technology, applied in the field of anemia detection, can solve the problems of unsatisfactory detection methods for sickle cell anemia and unsuitable for large-scale promotion and use, and achieve easy promotion, short detection time, fast and accurate detection Effect

Inactive Publication Date: 2016-02-03
BEIJING JINQI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is that the existing sickle cell anemia detection method is not ideal and is not suitable for large-scale promotion and use

Method used

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  • Gene detecting primers of sickle cell anemia and composition of gene detecting primers
  • Gene detecting primers of sickle cell anemia and composition of gene detecting primers
  • Gene detecting primers of sickle cell anemia and composition of gene detecting primers

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Embodiment Construction

[0038] In order to better understand the present invention, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0039] A gene detection primer for sickle cell anemia, comprising the following primers:

[0040] 5'-GTCGACTGTTGCTTACACTTTC-3' (SEQ ID No.1, hereinafter referred to as F3)

[0041] 5'-GCCCAGTTTCCATTTGCCT-3' (SEQ ID No.2, hereinafter referred to as B3)

[0042] 5′-TCTGGAGTCAGATGCACCATTCTGACATAACAGTGTTCACTAGC-3′ (SEQ ID No.3, hereinafter referred to as FIPA)

[0043] 5′-ACTGGAGTCAGATGCACCATTCTGACATAACAGTGTTCACTAGC-3′ (SEQ ID No.4, hereinafter referred to as FIPT)

[0044] 5'-TGAACGTGGATGCAGTTGGTGGGAGCCTCTCTTATAACCTTGATACC-3' (SEQ ID No. 5, hereinafter referred to as BIP).

[0045] A gene detection composition for sickle cell anemia, comprising the above primers, and one of dNTP, buffer, Bst polymerase, ultrapure water, indicator, betaine or dimethyl sulfoxide.

[0046] The composition is divided ...

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Abstract

The invention provides gene detecting primers of sickle cell anemia and a composition of the gene detecting primers and belongs to the technical field of anemia detection. The gene detecting primers and the composition thereof are characterized in that on the basis of LAMP reaction, the Allele-Specific LAMP technology is used for respectively designing specific primers aiming at the wild type and mutant type of the gene sequences of coding hemoglobin beta chains, and the genotypes related to the sickle cell anemia can be detected fast and accurately. The gene detecting primers and the composition thereof are low in cost, short in detecting time, high in accuracy and easy to popularize.

Description

technical field [0001] The invention relates to the technical field of anemia detection, in particular to a gene detection primer for sickle cell anemia and a composition thereof. Background technique [0002] Sickle cell anemia is an autosomal recessive genetic disease. The patient's red blood cells show a sickle shape under the microscope, and their oxygen-carrying function is only half of that of normal red blood cells. Under hypoxic conditions, this abnormal red blood cell Will rupture and cause severe anemia, and even cause death of the patient. Sickle cell anemia mainly occurs in Africans and African Americans, and cases have also been reported in southern my country in recent years. Molecular biology studies have found that the cause of the disease is that the gene sequence encoding the sixth amino acid of the hemoglobin β subunit is mutated from GAG to GTG, resulting in the mutation of the glutamic acid in the hemoglobin β chain to valine, thus causing Abnormal fun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q2531/119
Inventor 汪大为
Owner BEIJING JINQI BIOLOGICAL TECH CO LTD
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