Method for detecting polysaccharide content of ganoderma lucidum extract through near-infrared spectroscopy

Abstract

The invention discloses a method for detecting the polysaccharide content of a ganoderma lucidum extract through near-infrared spectroscopy. The method includes the following steps that firstly, a ganoderma lucidum extract sample is selected; secondly, near-infrared spectral acquisition is conducted; thirdly, a wave band area is selected; fourthly, spectrum data are preprocessed; fifthly, a prediction and correction model is optimized; sixthly, a PLS prediction and correction model is built; seventhly, the models are verified. By means of the method, the polysaccharide content of the ganoderma lucidum extract can be quickly and quantitatively detected, and detection time of each sample is within one minute. Under the significance level of 0.05, a prediction value and an actual measurement value almost have no significant difference, detection is accurate and reliable, the method can be directly applied to online detection of the polysaccharide content of the ganoderma lucidum extract, and the method has wide application and development prospects.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, relates to the field of rapid determination of polysaccharide content in edible fungus extracts, in particular to a rapid detection method for the polysaccharide content of Ganoderma lucidum extract with high nutritional value. Background technique [0002] China is rich in edible fungi resources, and it is also one of the first countries to cultivate and utilize edible fungi. Edible mushroom extracts are rich in protein and amino acids, and their content is several to dozens of times that of ordinary vegetables and fruits. Edible fungi extract is also rich in various mineral elements: phosphorus, potassium, sodium, calcium, iron, zinc, magnesium, manganese, etc. and other trace elements. Ganoderma lucidum extract has high medicinal value, and has significant effects in improving human non-specific immune function, anti-oxidation, anti-tumor, anti-radiation, anti-aging, lowering bl...

AI Technical Summary

Problems solved by technology

Visible spectrophotometer is mostly used to measure the polysaccharide content of Ganoderma lucidum, and its analysis steps are quite cumbersome. The reduction of the analyzed index components will affect the accuracy of the final results. The samples after routine physical and chemical analysis will be polluted to a certain extent, and the samples will not be able to continue to be used.

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