Kit for tracing uterus implanting stem cell prognosis, and preparation method thereof

A technology of stem cells and kits, applied in the field of obstetrics and gynecology, can solve the problems of limited tissue penetration and inability to display the spatial structure of organs, etc., achieve good biocompatibility, protect the welfare of experimental animals, and have a high degree of technology promotion Effect

Inactive Publication Date: 2016-03-02
THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

QDs have unique optical properties: strong fluorescence, good photochemical stability, less prone to photobleaching, fast and sensitive imaging, but limited tissue penetration, and cannot display the spatial structure of organs

Method used

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  • Kit for tracing uterus implanting stem cell prognosis, and preparation method thereof
  • Kit for tracing uterus implanting stem cell prognosis, and preparation method thereof
  • Kit for tracing uterus implanting stem cell prognosis, and preparation method thereof

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Embodiment Construction

[0026] Technical thinking of the present invention is:

[0027] Nanoparticles Superparamagnetic Iron Oxide Nanoparticles (SPIO@SiO 2 ) and near-infrared quantum dot silver sulfide (Ag 2 S-QDs) labeled umbilical cord mesenchymal stem cells (MSCs), the labeled stem cells were implanted into rat uterine injury wounds, and the implanted stem cells were checked by magnetic resonance and near-infrared quantum dot fluorescence imaging methods, and the kit was used to detect the damaged uterine repair implants. The fate of imported stem cells.

[0028] The present invention will be specifically described below.

[0029] 1: different orders of magnitude Ag 2 Near-infrared imaging observation of S-QDs-labeled MSCs combined with collagen scaffolds

[0030] In order to understand the cell distribution after stem cells were combined with collagen membranes, collagen membranes with cells of different orders of magnitude were placed in a petri dish and detected with a near-infrared secon...

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Abstract

The invention relates to kit for tracing uterus implanting stem cell prognosis, and a preparation method thereof. A technical scheme is characterized in that the kit comprises nano-particle superparamagnetic iron oxide nanoparticles and near infrared quantum dot silver sulfide labeled umbilical cord mesenchymal stem cells. The defects of long imaging time and limited susceptibility of a magnetic resonance in vivo imaging technology of superparamagnetic iron oxide nanoparticle labeled stem cells and the defects of limited tissue penetrating force and unable organ space structure display of a near infrared quantum dot fluorescence imaging technology are solved in the invention. Outward transfer positions of stem cell colonized tissues can be disclosed 1 week after body positioning stem cells to post-operation. Cell experiments of contrast agents SPIO@SiO2 and Ag2S-QDs prove that the biocompatibility is good, a magnetic resonance apparatus and a near infrared quantum dot fluorescence imager are commercial products, the technology can be highly popularized, and the technology is controllable, is highly safe, is beneficial to scientific researches of stem cell treatment of severe endometrium damages, and protects the welfware of experiment animals.

Description

technical field [0001] The invention belongs to the field of obstetrics and gynecology, and specifically relates to a kit for tracing the fate of stem cells implanted in the uterus and a preparation method thereof. Background technique [0002] Infertility etiology Thin endometrium and intrauterine adhesions are the restrictive factors affecting the success rate in the development of assisted reproductive technology. Uterine cavity transplantation of mesenchymal stem cells (MSCs) is a promising treatment method. The uterine wall injury of rats is treated with MSCs composite collagen scaffolds. The scaffolds provide structural support and the basis for cell adhesion, allowing a large number of cells to act as a living body. The tissue sheet is directly bonded to the damaged tissue, and the anatomical and functional restoration of the uterine injury has been achieved so far. However, the local location, differentiation and transfer of stem cells in the uterus after transplant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/44A61K49/08A61K49/00A61P15/00
Inventor 周白戴建武胡娅莉李新安曹昀
Owner THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
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