Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of salviae miltiorrhizae and flos carthami composition to enhancement of antithrombotic function of aspirin

A technology of aspirin and aspirin ester, applied in the field of traditional Chinese medicine composition, to achieve the effects of prolonging the action time, slowing down the hydrolysis rate, and enhancing the antithrombotic effect

Inactive Publication Date: 2016-03-23
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE +1
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: the technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and to provide a reliable curative effect through a large number of experimental studies, which can significantly reduce the activity of aspirin esterase, antagonize aspirin resistance, and enhance the antiplatelet effect of aspirin, thereby achieving Traditional Chinese medicine composition for enhancing antithrombotic effect of aspirin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of salviae miltiorrhizae and flos carthami composition to enhancement of antithrombotic function of aspirin
  • Application of salviae miltiorrhizae and flos carthami composition to enhancement of antithrombotic function of aspirin
  • Application of salviae miltiorrhizae and flos carthami composition to enhancement of antithrombotic function of aspirin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Effects of Danshen Safflower Compositions of Different Proportions and Formulations on the Antiplatelet Effect of Low-Dose Aspirin

[0024] 1. Experimental method

[0025] 1.1 Experimental materials

[0026] Salvia safflower composition injection: take different weight ratios of salvia miltiorrhiza and safflower (C1, 3 parts of salvia miltiorrhiza-1 part of safflower; C2, 1 part of salvia miltiorrhiza-1 part of safflower; C3, 1 part of salvia miltiorrhiza-3 parts of safflower ), add water to decoct twice, each time for 60 minutes, filter, combine and concentrate the filtrate to a clear paste with a relative density of 1.20-1.30 (65°C), add ethanol until the alcohol content is 75-80%, refrigerate, and take the supernatant, Recover ethanol and concentrate it into a clear paste with a relative density of 1.20-1.30, add ethanol until the alcohol content is 80-85%, refrigerate, take the supernatant, add about 1% of the input amount of activated carbon, stir for 30...

Embodiment 2

[0034] Example 2 Effect of Danshen Safflower Composition Injection on Aspirin Esterase Activity

[0035] 1. Experimental method

[0036] 1.1 Determination of plasma aspirin esterase activity

[0037]Add 100 μL blank plasma and 10 μL aspirin hydrochloride solution to 890 μL Tris buffer, vortex for 30 s, and incubate in a 37° C. water bath for 20 min. Take 100 μL of reaction solution and add 100 μL of internal standard solution to stop the reaction and precipitate a small amount of protein, centrifuge at 13000×g for 10 min, draw 150 μL of supernatant and inject. The concentration of salicylic acid was determined by UPLC-TQ-MS. The preparation method of the aspirin hydrochloric acid solution is as follows: 1.08 mg of aspirin is dissolved in 6 mL of 0.1 M hydrochloric acid (aspirin concentration is 1 mM). The preparation method of Tris buffer is: mix 0.2423g Tris (Tris) and 2.22g Calcium Chloride (CaCl 2 ) was dissolved in 100mL ultrapure water, and the pH was adjusted to 7.4 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a salviae miltiorrhizae and flos carthami composition to enhancement of the antithrombotic function of aspirin. On the basis of the traditional Chinese medicine prescription compatibility theory and a large number of experiments and researches, the salviae miltiorrhizae and flos carthami composition is screened out and can remarkably inhibit the esterase activity of aspirin, the hydrolysis speed of aspirin is reduced, the acting time of aspirin is prolonged, aspirin resistance is inhibited, the antiplatelet function of aspirin is enhanced, the antithrombotic treatment effect can be enhanced by clinically using the salviae miltiorrhizae and flos carthami composition and aspirin, adverse effects are reduced, and application prospects are important.

Description

technical field [0001] The invention relates to a new application of a traditional Chinese medicine composition, in particular to the application of the Danshen safflower composition in enhancing the antithrombotic effect of aspirin, inhibiting the activity of aspirin esterase and promoting the antiplatelet effect of aspirin. Background technique [0002] Aspirin is a classic antipyretic, analgesic and anti-inflammatory drug, and is one of the most widely used drugs in the world. The current main use of aspirin is to prevent and treat cardiovascular diseases in small doses. [0003] During the absorption process, aspirin is partially hydrolyzed into salicylic acid through the first-pass effect of the gastrointestinal tract and the first-pass effect of the liver, and aspirin (50-70%) entering the blood circulation as a prototype is rapidly hydrolyzed into water by aspirin esterase in the liver and blood Salicylic acid. Aspirin can acetylate the COX-1 enzyme active center of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61P7/02A61K31/616
Inventor 段金廒赵步长唐志书李建萍郭建明钱大玮赵菁
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com