A digital pcr absolute quantitative typing detection kit for detecting hbv-b/c

Abstract

The invention discloses a typing detecting kit and method for detecting the digital PCR absolute quantification of HBV-B / C. The kit comprises a DNA extraction solution, a digital PCR reaction buffer solution A, a digital PCR reaction buffer solution B, an HBV-B virus gene positive quality control, an HBV-C virus gene positive quality control, a negative quality control and an internal standard solution. The method includes the following steps of firstly, processing a to-be-detected sample; secondly, processing a quality control product; thirdly, preparing a digital PCR reaction mixed solution; fourthly, generating a micro-reaction liquid drop, and conducting digital PCR reaction amplification; fifthly, reading a fluorescent signal, analyzing an HBV type and calculating the number of copying times. By means of the digital PCR technology and the dual-color florescent probe, two subtypes of HBV are detected at the same time; without depending on an external standard object or a standard curve, the kit and method are easy and convenient to operate and can directly detect the HBV precise absolute quantification.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnostic biology, and in particular relates to a digital PCR absolute quantitative typing detection kit for detecting HBV-B / C and a detection method thereof. Background technique [0002] Hepatitis B virus is the pathogen that causes acute and chronic hepatitis B in humans. It can cause serious damage to liver cells, and persistent and stable HBV infection is closely related to the occurrence of liver cirrhosis and liver cancer, which is extremely harmful to human health. It is estimated that there are about 2 billion people who have been infected with HBV in the world, and more than 350 million infected people have persistent stable and chronic infections; the number of deaths from related diseases caused by HBV infection can reach 600,000-1 million every year. The prevalence of HBV infection varies widely around the world, with the highest prevalence in eastern Asia and low prevalence in nor...

AI Technical Summary

Problems solved by technology

[0006] For the above-mentioned deficiencies that exist in the prior art, the technical problem to be solved in the present invention is: how to provide a kind of digital PCR absolute quantitative typing detection kit and detection method thereof for detecting HBV-B / C, for overcoming existing In the detection technology, the steps are complicated, time-consuming, easily lead to cross-hybridization reaction, quantitative correction is required, and the accuracy is not high, etc., and it has the characteristics of high accuracy, high sensitivity, high precision, and good reproducibility

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