Tissue-culture rapid propagation method for arundina graminifolia

A technology for tissue culture and rapid propagation and bamboo leaf orchid, which is applied in the field of plant tissue culture, can solve the problems such as inability to obtain genetic material seedlings, easy separation of traits, etc., and achieves easy establishment of sterile lines, shortening induction time, and improving production efficiency. and profit effects

Inactive Publication Date: 2016-04-13
三明市农业科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the test-tube plantlets obtained by aseptic sowing of seeds are seedlings, and their cha

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The method for rapid propagation of bamboo leaf orchid tissue culture of the present invention comprises the following steps:

[0021] Step 1 Explant material selection and surface disinfection:

[0022] Take high-position buds that are more than 10 cm away from the upper surface of the cultivation medium and full of high-position buds with a length of 3-5 cm as the selection material, cut off the high-position buds as explants, first rinse them under tap water, and then under sterile conditions, use 70 Disinfect with -75% alcohol for 30-60 seconds, rinse once with sterile water, then disinfect with 0.1% mercury liter for 8-12 minutes, rinse with sterile water for 3-5 times, and blot dry with sterile filter paper for later use.

[0023] Induction culture of step 2 adventitious buds:

[0024] Under sterile conditions, first cut off the top 1-3 cm of the sterilized high buds, and then inoculate the remaining part with the plant growth regulator 6-BA3.0mg / L+NAA0.1mg / L wit...

Embodiment 2

[0032] Step 1 Explant material selection and surface disinfection:

[0033] Take high-position buds that are more than 10 cm away from the upper surface of the cultivation medium and full of high-position buds with a length of 3-5 cm as the selection material, cut off the high-position buds as explants, first rinse them under tap water, and then under sterile conditions, use 70 Disinfect with -75% alcohol for 30-60 seconds, rinse once with sterile water, then disinfect with 0.1% mercury liter for 8-12 minutes, rinse with sterile water for 3-5 times, and blot dry with sterile filter paper for later use.

[0034] Induction culture of step 2 adventitious buds:

[0035] Under sterile conditions, first cut off the top 1-3 cm of the sterilized high buds, and then inoculate the remaining part with the plant growth regulator TDZ0. On the MS medium, culture in the cultivation room under light, keep the light intensity 1500-2000LX, the light time is 12 hours a day, the temperature is 2...

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PUM

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Abstract

A tissue-culture rapid propagation method for arundina graminifolia is characterized by comprising step 1, selecting and disinfecting explant materials, concretely selecting a high bud as an explant, and performing disinfection for usage; step 2, performing inducing culture to obtain adventitious bud, concretely, cutting the top of the high bud, inoculating an adventitious bud culture medium with the high bud, so as to obtain the adventitious bud through inducing; step 3, performing propagation culture to clustered buds, concretely, inoculating a clustered-bud induction medium with the adventitious bud, so as to obtain the clustered buds, and then inoculating a propagation medium with the clustered buds, so as to obtain a clustered-bud block mass; step 4, performing seedling strengthening and rooting culture, concretely, cutting the large clustered-bud block mass obtained in the step 3 into small block masses, then inoculating the seedling-strengthening rooting medium with the small clustered-bud block masses obtained in the step 3, wherein the small clustered-bud block mass grow vigorously and new roots are grown; and step 5, performing seedling hardening and domestication transplanting, when a seedling grows to 4-8 cm and has 1-3 roots, performing seedling-hardening culture, then taking the seedling out, immersing in a thiophanate methyl solution, and finally transplanting by using moss. The variation probability of arundina graminifolia during subculture proliferation is reduced.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rapid propagation of bamboo leaf orchid tissue culture. Background technique [0002] Aluminagraminifolia (Aluminagraminifolia Hochr) is a perennial herbaceous plant of the Orchidaceae Alumina genus, mainly distributed in Southeast Asia, and produced in Fujian, Jiangxi, Zhejiang, Guangdong, Hainan, Guangxi, Sichuan, Guizhou, Hunan, Yunnan and other places in China. Its flowers are beautiful and elegant, with light fragrance, long flowering period and high ornamental value. It can be potted or used for garden greening. Bamboo leaf orchid also has high medicinal value, and the whole plant is used as medicine. It has the effects of regulating qi and blood, clearing away heat and detoxification, dispelling rheumatism, anti-inflammatory and diuretic. It can be used to treat rheumatic low back pain, stomach pain, urinary tract infection, poisonous snake bite...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 周辉明林辉锋尚伟陈昌铭江秋萍邓才生林发壮叶榕妹王雪凤夏朝水魏柳萍梁泉生姚凤琴
Owner 三明市农业科学研究院
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