Tissue-culture rapid propagation method for arundina graminifolia

Abstract

A tissue-culture rapid propagation method for arundina graminifolia is characterized by comprising step 1, selecting and disinfecting explant materials, concretely selecting a high bud as an explant, and performing disinfection for usage; step 2, performing inducing culture to obtain adventitious bud, concretely, cutting the top of the high bud, inoculating an adventitious bud culture medium with the high bud, so as to obtain the adventitious bud through inducing; step 3, performing propagation culture to clustered buds, concretely, inoculating a clustered-bud induction medium with the adventitious bud, so as to obtain the clustered buds, and then inoculating a propagation medium with the clustered buds, so as to obtain a clustered-bud block mass; step 4, performing seedling strengthening and rooting culture, concretely, cutting the large clustered-bud block mass obtained in the step 3 into small block masses, then inoculating the seedling-strengthening rooting medium with the small clustered-bud block masses obtained in the step 3, wherein the small clustered-bud block mass grow vigorously and new roots are grown; and step 5, performing seedling hardening and domestication transplanting, when a seedling grows to 4-8 cm and has 1-3 roots, performing seedling-hardening culture, then taking the seedling out, immersing in a thiophanate methyl solution, and finally transplanting by using moss. The variation probability of arundina graminifolia during subculture proliferation is reduced.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rapid propagation of bamboo leaf orchid tissue culture. Background technique [0002] Aluminagraminifolia (Aluminagraminifolia Hochr) is a perennial herbaceous plant of the Orchidaceae Alumina genus, mainly distributed in Southeast Asia, and produced in Fujian, Jiangxi, Zhejiang, Guangdong, Hainan, Guangxi, Sichuan, Guizhou, Hunan, Yunnan and other places in China. Its flowers are beautiful and elegant, with light fragrance, long flowering period and high ornamental value. It can be potted or used for garden greening. Bamboo leaf orchid also has high medicinal value, and the whole plant is used as medicine. It has the effects of regulating qi and blood, clearing away heat and detoxification, dispelling rheumatism, anti-inflammatory and diuretic. It can be used to treat rheumatic low back pain, stomach pain, urinary tract infection, poisonous snake bite...

AI Technical Summary

Problems solved by technology

However, the test-tube plantlets obtained by aseptic sowing of seeds are seedlings, and their characters are prone to separation, so seedlings with consistent genetic material cannot be obtained.