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Fingerprint detection method for Shenshuaining granule

A detection method and fingerprint technology, which is applied in the field of fingerprint detection of Shenshuaining granules, can solve the problems of inability to systematically and comprehensively reflect the inherent quality, type and content of Shenshuaining granules, and achieve good absorption and baseline Smooth, good separation effect

Active Publication Date: 2016-04-13
山西德元堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because Shenshuaining Granules contain ten flavors of Chinese herbal medicines, the quality of Chinese herbal medicines is affected by many aspects such as the place of origin, climate, season, etc., resulting in the type and content of the ingredients contained are often unstable. The Chinese medicine compound only detects the content of a single ingredient, which is somewhat one-sided.
Therefore, none of the above methods can systematically and comprehensively reflect the intrinsic quality of Shenshuaining Granules.

Method used

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  • Fingerprint detection method for Shenshuaining granule
  • Fingerprint detection method for Shenshuaining granule
  • Fingerprint detection method for Shenshuaining granule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The Shenshuaining particle fingerprint detection method described in the present embodiment comprises the following steps:

[0049] a. Determine the chromatographic conditions

[0050] Chromatographic column: Agilent SB-C18 chromatographic column, the column specification is 5μm, 4.6mm×250mm;

[0051] Mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.1% formic acid;

[0052] Detection wavelength: 280nm;

[0053] Column temperature: 20°C;

[0054] Volume flow: 1mL / min;

[0055] Injection volume 10μL;

[0056] Elution program: Gradient elution, the elution time is 90min, the specific elution program is:

[0057] time min

Mobile phase A%

Mobile phase B%

0

5

95

5

5

95

4 --> 12

13

87

55

28

72

70

65

35

80

100

0

90

100

0

[0058] b. Preparation of the test solution: Accurately weigh 0.5g of Shenshuaining granule powder, add 25mL of...

Embodiment 2

[0080] The Shenshuaining particle fingerprint detection method described in the present embodiment comprises the following steps:

[0081] a. Determine the chromatographic conditions

[0082] Chromatographic column: Agilent SB-C18 chromatographic column, the column specification is 5μm, 4.6mm×250mm;

[0083] Mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.1% formic acid;

[0084] Detection wavelength: 285nm;

[0085] Column temperature: 30°C;

[0086] Volume flow: 0.8ml / min;

[0087] Injection volume: 20μL;

[0088] Elution program: Gradient elution, the elution time is 90min, the specific elution program is:

[0089] time min

Mobile phase A%

Mobile phase B%

0

5

95

12

13

87

55

28

72

70

65

35

75

100

0

90

100

0

[0090] b. Preparation of the test solution: Accurately weigh 1 g of Shenshuaining granule powder, add 80 mL of 70% methanol, ultrasonic...

Embodiment 3

[0094] The Shenshuaining particle fingerprint detection method described in the present embodiment comprises the following steps:

[0095] a. Determine the chromatographic conditions

[0096] Chromatographic column: Agilent SB-C18 chromatographic column, the column specification is 5μm, 4.6mm×250mm;

[0097] Mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.1% phosphoric acid;

[0098] Detection wavelength: 254nm;

[0099] Column temperature: 20°C;

[0100] Volume flow: 1.2ml / min;

[0101] Injection volume: 5 μL;

[0102] Elution program: Gradient elution, the elution time is 90min, the specific elution program is:

[0103] time min

Mobile phase A%

Mobile phase B%

0

5

95

12

13

87

60

28

72

75

65

35

80

100

0

90

100

0

[0104] b. Preparation of the test solution: Accurately weigh 1 g of Shenshuaining granule powder, add 100 mL of 50% methanol, ultra...

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Abstract

The invention relates to a fingerprint detection method for Shenshuaining granule. The detection method employs HPLC for detection, and comprises the following steps: a, determining chromatographic condition; b, preparing a tested object solution; and c, establishing fingerprint. The chromatographic conditions in the step comprise that the chromatographic column is C18 chromatographic column; the mobile phase comprises a mobile phase A and a mobile phase B with the ratio of 5%:95%-100%:0%, the mobile A is acetonitrile or methanol, and the mobile phase B is water, a phosphoric acid aqueous solution with the concentration of 0.01%-0.5% or a formic acid aqueous solution with the concentration of 0.01%-0.5%; the detection wave length is 190-450 nm; the column temperature is 20-40 DEG C; the volume flow is 0.8-1.2 mL / min; the sample size is 5-100 mu L; and the elution program employs gradient elution and the elution time is 80-100 min. The detection method is simple, rapid, easy to operate, high in precision, good in stability and good in reappearance, possesses many characteristic peaks, and can well provide quality control basis for production of Shenshuaining granule.

Description

technical field [0001] The invention belongs to the technical field of detecting the quality of traditional Chinese medicine preparations by means of modern analysis and detection means and information processing means, and relates to a detection method for establishing the HPLC-UV standard characteristic fingerprint of Shenshuaining granules by high performance liquid chromatography. Background technique [0002] Shenshuaining Granules are composed of ten herbs including Danshen, Rhubarb, Coptidis, Pseudostellaria Pseudostellaria, Pinellia (made), Chenpi, Poria, Achyranthes bidentata, safflower and licorice. The effect of excreting turbidity. It is used for spleen failure, turbid stasis, low back pain, fatigue, sallow complexion, nausea and vomiting, loss of appetite, dysuria, viscous stool and chronic renal insufficiency caused by various reasons. [0003] The composition of traditional Chinese medicine is complex, and the active part is often not a single component. Taki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 段陈平路金才刘建丽吕重宁
Owner 山西德元堂药业有限公司
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