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Kit for rapid detection of Mycobacterium tuberculosis in sputum

A technology of mycobacterium tuberculosis and a kit, which is applied in the biological field, can solve the problems of long time consumption, low sensitivity of antibody detection and false positives, etc., and achieve the effects of short time consumption, reduced experiment time, and easy operation

Inactive Publication Date: 2016-04-27
SHANGHAI XINGYAO MED TECH DEV CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

[0009] Due to the low sensitivity of antibody detection and many false positives, nucleic acid detection requires large-scale instruments such as fluorescent PCR instruments, and the nucleic acid extraction of pathogenic bacteria is required in advance, and subsequent fluorescent detection takes a long time. One instrument is widely used, cheap and easy to operate. A simple, intuitive, and highly sensitive detection method is on the horizon

Method used

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  • Kit for rapid detection of Mycobacterium tuberculosis in sputum

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Embodiment 1

[0037] The detection of mycobacterium tuberculosis in the sputum sample of embodiment 1

[0038] Detection method:

[0039] (a) Take 500ul of the sputum sample to be tested, add it to 1ml of liquefaction solution, shake and mix;

[0040] (b) Take 10ul of the liquid obtained by step (a) and drop it on the glass slide, place it on a heating plate and dry it at 52°C for 5min;

[0041] (c) Immerse the glass slide obtained in step (b) in ethanol for 5 minutes, place it on a heating plate and dry it at 52°C for 5 minutes;

[0042] (d) Add 10 ul of human probes to the sample wells of the glass slide obtained through the operation in step (c), place on a heating plate and dry at 52°C in the dark for 5 minutes;

[0043] (e) Immerse the glass slide obtained in step (d) in the stop solution for 40 sec, place it on a heating plate and dry it at 52°C for 5 min;

[0044] (f) Add 15ul of fixative solution to the sample hole of the slide obtained in step (e), cover with a cover glass, obse...

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Abstract

The present invention provides a kit for rapid detection of Mycobacterium tuberculosis in a sputum sample, and the kit includes a liquefied solution, a lysis solution, a probe solution, a stop solution, a fixative solution, a carrying glass sheet and a cover glass sheet. After liquefaction of the sputum sample, cell lysis, probe hybridization and fluorescence fixing and other operations are performed, and a fluorescence microscopy is used for macroscopic observation, counting and analysis of the Mycobacterium tuberculosis. The kit is simple in operation method, short in procedure, easy to operate, strong in test result specificity, high in sensitivity, clear in results and high in reliability, and can be used for direct, simple and effective detection of the Mycobacterium tuberculosis in the sputum sample.

Description

technical field [0001] The invention belongs to the field of biological technology and relates to a biological detection technology, in particular to a kit for rapidly detecting mycobacterium tuberculosis in sputum samples. Background technique [0002] Mycobacterium tuberculosis, commonly known as Mycobacterium tuberculosis, is the pathogenic bacterium that causes tuberculosis, which can invade various organs of the body, but tuberculosis is the most common. Tuberculosis is still an important infectious disease. According to the WHO, about 8 million new cases occur each year and at least 3 million people die from the disease. Before the founding of the People's Republic of my country, the mortality rate reached 200-300 people / 100,000, ranking first among the causes of death from various diseases. Morbidity and mortality are greatly reduced. However, it should be noted that in some areas of the world, the incidence rate is on the rise again due to AIDS, drug abuse, applica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/32
CPCC12Q1/6841
Inventor 迟大利吴大治夏懿
Owner SHANGHAI XINGYAO MED TECH DEV CO LTD
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