Simple method using bombyx mori common speckle breed to form practical tea speckle sex-limiting system
A practical, varietal technology, applied in the field of plaque-limited systems, that can solve problems such as genetic difficulties
Active Publication Date: 2016-05-04
湖南省蚕桑科学研究所 +1
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Problems solved by technology
[0005] The technical problem to be solved by the present invention is the genetic difficulty in constructing a practical tea spot-limited system using practical common spot varieties, so as to achieve continuous backcrossing of practical common spot varieties and quickly make new breeding tea spot-limited system materials Contains more practical bloodlines
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[0012] like figure 1 Shown, a kind of method of the present invention utilizes silkworm common stripe variety to construct practical tea spot limited system,
[0013] Hybridization: limited variety ZW^ based on basic markings + p / p+ L / + L (Dan Pu Ban, ♀) is the female parent, practical Pu Ban variety ZZ+ / ++ L / + L (Pu Ban, ♂) is the male parent, crossed to get F 1 Generation, select F 1 The female ZW^ in the generation + + / p+ L / + L F 2 ;
[0014] Continuous backcrossing: visual removal of F 2 Medium and deep Pucha individuals, female individuals ZW^ were selected for pupa identification + + / pL / + L (Pu Cha, ♀) and practical Pu Ban variety ZZ+ / ++ L / + L (Reincarnation parent, Pu Ban, ♂) male backcross, BC 1 generation to BC n-1 In each generation of backcross, the non-tea spot individuals and the deep tea spot individuals in the offspring were continuously eliminated, and the female individuals ZW^ were selected for pupa identification. + + / pL / + L (Pu Cha, ♀...
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The invention discloses a simple method using bombyx mori common speckle breeds to form a practical tea speckle sex-limiting system; the method comprises the following steps: using a basic speckle sex-limiting breed as female parent and a practical common speckle breed as male parent, and crossbreeding the two so as to obtain F1; selecting F1 female and basic common tea breed male for hybridization so as to obtain F2; using naked eyes to remove deep common tea individuals in F2, selecting pupa female individuals to backcross with the practical common speckle breed, from BC1 to backcross each generation to contentiously weed out non-tea speckle individuals and deep common tea individuals in tea speckle from after generations, and selecting pupa female individuals for backcrossing; using same method to weed out after generation individuals from BCn generation, separating common tea from light common tea individuals for feeding, selecting pupa female from common tea breed so as to inbreed with light common tea, and F3 selects female from separately feeding light common tea for inbreeding with Ji tea male; F4 selects non-tea speckle individuals for inbreeding, and F5 weeds out moth zone having non-tea speckle individuals until no non-tea speckle individual appear. The novel method can solve the problems that common speckle breed cannot contentiously backcross and regenerate, is simple in operation, and fast in process.
Description
technical field [0001] The invention relates to the field of genetics and breeding of silkworms, in particular to a simple and convenient method for constructing a practical tea spot-limited system by using ordinary spotted silkworm varieties. Background technique [0002] At present, the stripe-limited variety is one of the most successfully applied labor-saving silkworm varieties. In this type of silkworm variety, the larvae with stripes are female, and the larvae without stripes are male. It can be used according to The presence or absence of larval markings, the sex of different individuals can be identified with the naked eye, and the traditional process of artificially "identifying pupae" to distinguish males and females at the pupa stage during the breeding process of silkworm eggs at all levels can reduce the labor cost of silkworm egg breeding by more than 20%. However, this type of species can only be divided into small silkworms when the larvae develop to the larg...
Claims
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IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 艾均文鲁成代方银何行健薛宏刘昌文刘勇郑颖肖建清李飞鸣
Owner 湖南省蚕桑科学研究所