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Method for creating germ plasma through distant composite hybridization of tuber mustard and radishes

A technology of compound hybridization and mustard, applied in the field of biological genetics, can solve the problems of high incidence frequency, large incidence area, difficult prevention and control, etc., and achieve the effects of less incidence, promotion of replacement, and poor resistance to stress.

Active Publication Date: 2016-05-11
重庆市渝东南农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Stem mustard virus disease is a historical epidemic disease of mustard raw material plants. Because of its high frequency and large area of ​​occurrence, the loss is serious after occurrence, and it is difficult to control after occurrence.

Method used

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  • Method for creating germ plasma through distant composite hybridization of tuber mustard and radishes
  • Method for creating germ plasma through distant composite hybridization of tuber mustard and radishes
  • Method for creating germ plasma through distant composite hybridization of tuber mustard and radishes

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A method for creating germplasm by distant compound hybridization of pickled mustard and radish, the operation steps of which are as follows:

[0032] (1) Hybridization:

[0033] A, the selection of parents: choose well-developed, strong, no pests and diseases early-maturing mustard variety Linshicaoyaozi, mid-late-maturing varieties Suanye and late-maturing varieties Yubo, plants in the blooming period, as the female parent, choose well-developed, robust, non-toxic The radish with good resistance to diseases and insect pests is used as the male parent;

[0034] a) Treat the female parent: ① Arranging the inflorescence: remove the buds that have already bloomed, the yellow buds that are about to bloom, and the buds that are too small at the top, and only keep 20-25 buds in the middle, and then use tweezers to remove the buds that develop in the middle Bad flower buds; ②Detasselling: clamp the whole inflorescence between the thumb and middle finger of the left hand, wit...

Embodiment 2

[0047] The difference from Example 1 is only that: in step (1), the selected variety of mustard mustard female parent is: mid-ripening variety Yongan Xiaoye; Linshi grass kidney, all the other operations are consistent with embodiment 1.

Embodiment 3

[0049] The only difference from Example 1 is that the selection of mustard mustard female parent in step (1) is: mid-ripening mustard variety Pixian mustard; in step (4), other mustard varieties backcrossed are: semi-broken leaves, Yongan small leaves and Linshi grass kidney, all the other operations are consistent with embodiment 1.

[0050] It can be seen from this: by crossing a mustard variety with strong combining ability with radish to obtain distant hybrid offspring material, the genetic material of radish is introduced into mustard variety, and then the distant hybrid offspring material is used as an intermediate bridge to carry out distant hybridization. A large number of new germplasm materials of different mustard varieties and radish were obtained by compound crossing. At the same time, this intermediate bridge can be further hybridized with radish, and then hybridized with mustard to introduce the good genes of radish into mustard. And at the same time, this inte...

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Abstract

The invention discloses a method for creating germ plasma through distant composite hybridization of tuber mustard and radishes. The method includes the following operating steps of 1, hybridization, wherein three tuber mustard varieties with early, middle and late maturing characteristics are selected as female parents, the radishes serve as male parents, artificial hybridization is conducted, and a generation F1 is obtained; 2, selfing, wherein the generation F1 is subjected to selfing, separation and seed reserving, and a generation F2 with four express characters is obtained; 3, composite crossing, wherein the generation F1 is taken as a female parent, the tuber mustard varieties are selected as male parents, composite crossing is conducted, and a three-way cross hybrid is obtained; 4, back crossing, wherein the three-way cross hybrid serves as a female parent, the tuber mustard varieties serving as the male parent in the step 3 are selected as back crossing male parents, continuous back crossing is conducted, and a stably-genetic distant hybridization filial generation is obtained. The method for creating the germ plasma through distant composite hybridization of the tuber mustard and the radishes can obtain stable genetic resources, and is simple, rapid and efficient.

Description

technical field [0001] The invention relates to the field of biological genetics, in particular to a method for creating germplasm by compound hybridization of pickled mustard and radish. Background technique [0002] Mustard mustard, as a special product of our country, has been widely welcomed by domestic and foreign consumers for a long time. Especially Chongqing mustard, represented by "Fuling mustard", enjoys a high reputation at home and abroad because of its unique quality of "fresh, fragrant, tender and crisp", rich nutrition, convenience and deliciousness, and storage and cooking resistance. Cucumbers and Japanese pickles are also known as the world's three famous pickles. Chongqing is the birthplace of pickled mustard production, and it is also the main production area of ​​mustard mustard with the largest production and processing scale and the most concentrated planting in my country. Mustard mustard industry has become one of the most characteristic and advant...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 沈进娟范永红林合清王旭祎刘义华吴朝君冉广葵赵守忠
Owner 重庆市渝东南农业科学院
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