Direct quantification of unprocessed nucleic acid samples

A nucleic acid, direct technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of time and resource consumption, lack of availability, etc.

Active Publication Date: 2020-05-29
LIFE TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These procedures limit any value of real-time quantitative analysis as a screening tool, because by the time the sample is ready for real-time quantitative analysis, substantial time and resources have been spent extracting and purifying the sample, which may not have any evidentiary value

Method used

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  • Direct quantification of unprocessed nucleic acid samples
  • Direct quantification of unprocessed nucleic acid samples
  • Direct quantification of unprocessed nucleic acid samples

Examples

Experimental program
Comparison scheme
Effect test

example

[0099] PE swab sample collector

[0100] An example of a PE swab used to collect samples for analysis is depicted in figure 1 middle. The PE swab was assembled by wrapping a filter paper strip around the holder, and the paper strip was then fastened to the holder by a clip at the end of the handle of the PE swab. The width of the active sampling area is defined by the angled folds of the holder. exist figure 1 In the example shown in , the PE swab is about 40mm long when measured from the active sampling area to the end of the swab handle. In this example, the active sampling area is about 5mm. The PE swab handle is about 15mm wide.

[0101] The active sampling area of ​​a 5mm PE swab is approximately 1mm x 5mm. After removal of the 0.5 mm punch, four 1 mm punches can actually be produced from the remaining active sampling area of ​​the 5 mm PE swab.

[0102] In some cases, a liquid is applied to the surface to facilitate sample collection by swabbing.

[0103] After w...

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PUM

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Abstract

A workflow for direct qPCR quantification of unprocessed forensic case samples is disclosed herein. 13 pg of DNA have been detected by direct amplification from paper substrates. Direct qPCR quantification of unprocessed forensic case samples and direct STR amplification of unprocessed forensic case samples collected on the same PE swabs will greatly increase the efficiency and capacity of forensic laboratories.

Description

Background technique [0001] Increased recognition of the ability of DNA technology to be used to solve cases has significantly increased the demand for DNA analysis. Law enforcement agencies that used to collect DNA evidence only in violent crimes, such as homicide and sexual assault, have begun collecting DNA evidence from property crime cases to aid their investigations. However, biological evidence samples from property crimes are mostly touch DNA samples. Unlike blood or saliva stains, touch DNA isn't always detectable by the naked eye. In many cases, law enforcement officers are at crime scenes swabbing surfaces they believe have been touched by the perpetrator or collecting evidence they believe had been in physical contact with the perpetrator. It is therefore understandable that some samples collected in this manner may not contain any DNA at all. Even when perpetrator DNA is collected on a swab, there is no guarantee that it will contain enough DNA to obtain a prov...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6806C12Q1/686
CPCC12Q1/6806C12Q2561/113C12Q2565/607C12Q2565/625C12Q1/686C12Q1/6851
Inventor J·Y·刘
Owner LIFE TECH CORP
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