Method for rapidly identifying cashmere cycle development, molecular target and applications thereof

A molecular target, cashmere technology, applied in the field of molecular targets, can solve the problems of less research on skin and hair follicles, not realizing the importance, and no miRNA, etc.

Inactive Publication Date: 2016-06-08
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] When researchers first discovered miRNA two decades ago, they didn't realize its importance
[0008] Although the role of miRNA on skin hair follicles is currently less studied
There are no reports of miRNA targets in cashmere cycle development

Method used

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  • Method for rapidly identifying cashmere cycle development, molecular target and applications thereof
  • Method for rapidly identifying cashmere cycle development, molecular target and applications thereof
  • Method for rapidly identifying cashmere cycle development, molecular target and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0049] The beneficial effects of the present invention will be further described below in conjunction with the specific embodiments given by the inventor, rather than limiting the present invention.

[0050] 1. Materials and databases:

[0051] miRNA database: miRbase (http: / / www.miRbase.org / )

[0052] miRNA comparison: tag2miRNA (developed by BGI)

[0053] Homology comparison software: BLAST (http: / / blast.ncbi.nlni.nih.gov / Blast.cgi)

[0054] Candidate miRNA prediction: Mireap (http: / / sourceforge.net / projects / mireap / )

[0055] Primer online design software: primer3 (http: / / frodo.wi.mit.edu / primer3 / )

[0056] Unless otherwise specified, the methods used in the following examples are conventional methods, and the reagents and instruments used can be obtained from commercial sources.

[0057]

[0058] serial number

instrument

manufacturers

1

Centrifuge 5415D

Eppendorf

2

Centrifuge 5810R

Eppendorf

3

Spectrophotomet...

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PUM

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Abstract

The invention discloses a method for rapidly identifying cashmere cycle development. The goat miRNA is identified comprehensively, the differential expression profiles of goat skin follicle in different growth periods (growth period, rest period, and regression period) are compared so as to screen out miRNA of differential expression; a technology of fluorescent quantitation is adopted to verify the result; through target gene prediction and transcript profile difference comparison, the gene for modulating follicles is searched, the functions of miRNA is determined; and finally the miRNA-263 is the specific miRNA for cycle development of follicles. Through identifications, people find that the miR-263b is accord with the cashmere cycle development rule; the target gene modulated by miR-263b is part of key gene in the cashmere cycle development pathway; the change of expression of miR-263b can precisely represents the specific periods of cashmere, and thus miR-263b can be used to indicate the periods. So miR-263b has an important value on research and application, and can be used as a novel reference for prognosis judgment of cashmere cycle development.

Description

technical field [0001] The invention belongs to the technical field of molecular targets, and relates to a method for rapidly identifying the cycle development of cashmere and its molecular targets and applications. Background technique [0002] Inner Mongolia cashmere goats and cashmere occupy an important position in the world, are valuable germplasm genetic resources, and are the most internationally competitive industry in animal husbandry. Inner Mongolia cashmere goats are famous all over the world for their slender, white, soft and shiny cashmere. In 2008, there were 18.68 million cashmere goats in stock, and 7,642 tons of cashmere were produced, accounting for 48% of the national cashmere production and 30% of the world's cashmere production. The cashmere enterprises in the autonomous region have an annual sales income of 16 billion yuan. The cashmere goat industry has become an important national and people's livelihood industry in the Inner Mongolia Autonomous Regi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
Inventor 刘志红肖红梅王志新李金泉
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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