PCR primer sets, probe sets and kits for detecting various respiratory pathogens

Abstract

The invention discloses a PCR (polymerase chain reaction) primer group, a probe set and a kit for detecting multiple respiratory pathogens. Sequences of PCR primers are represented as SEQ ID NO.1-SEQ ID NO.12; probes comprise at least two of six molecular beacon probes for respiratory pathogens, a fluorescence group and a quenching group are formed at two ends of each molecular beacon probe respectively, and sequences are represented as SEQ ID NO.13-SEQ ID NO.18. The invention further discloses a PCR reaction liquid containing the PCR primers and the probes as well as a kit containing the PCR reaction liquid for at least one person. With the adoption of the PCR primer group, the probe set and the kit, the six respiratory pathogens can be rapidly and accurately detected in a typing manner.

Description

technical field [0001] The invention relates to a PCR primer set, a probe set and a kit for detecting various respiratory pathogens. Background technique [0002] Respiratory tract infection is one of the most common clinical diseases, which can be caused by a variety of pathogens, including bacteria, viruses, mycoplasma, chlamydia, etc. Among them, acute respiratory tract infection (Acute Respiratory Tract Infection, ARTI) is an important cause of illness and death in children worldwide. The World Health Organization (WHO) published an article in the "Lancet" magazine in 2005 and reported that in 2000-2003, among the 10.6 million children under the age of 5 who died each year, 54% of them died from infectious diseases, of which 54% died from pneumonia. Children accounted for 19%. ARTI is strictly defined as all respiratory infections, however, in reality, the acute lower respiratory tract accounts for the majority of severe respiratory diseases, accounting for 20% of all ...

AI Technical Summary

Problems solved by technology

[0004] With the development of the medical level, the above-mentioned traditional methods can no longer satisfy the detection of respiratory pathogens. Clinically, there is a need for a sensitive, specific, fast and simple method for the identification and detection of respiratory multi-pathogens, in order to be able to do better. Individualized treatment, and meet the needs of sanitation and epidemic prevention and epidemic control, and epidemiological research on infectious diseases

Images