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A lung cancer screening kit

A reagent and technology for lung cancer, applied in the field of lung cancer screening kits, has achieved good clinical application prospects

Active Publication Date: 2018-09-14
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the relationship between plasma HSP60 and lung cancer

Method used

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  • A lung cancer screening kit
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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The relationship between embodiment 1 HSP60 expression level and lung cancer

[0022] The experimental method is as follows:

[0023] 1. Clinical data

[0024] Fifteen patients with early lung cancer and 10 healthy controls were selected. The basic information is as follows:

[0025] Basic Information

lung cancer patients

healthy control

number of people

15

10

age

61.2±12.2

49.5±10.6

male ratio

10(66.7％)

6(60.0％)

[0026] 2. Detection of HSP60 expression level (quantitative detection of HSP60 content)

[0027] Using the AAH-BLG-CUST kit purchased from RayBiotech (article number: AAH-BLG-CUST), the expression level of HSP60 was detected according to the following method:

[0028] 1. Sample dialysis

[0029] Extract blood from lung cancer patients and healthy controls, centrifuge after EDTA anticoagulation (1200rpm, 10min), absorb the supernatant and perform a second centrifugation (3000rpm, 15min), absorb t...

Embodiment 2

[0059] Embodiment 2 Composition and method of use of the kit for detecting the expression level of HSP60 in blood according to the present invention

[0060] 1. The composition of the HSP60 detection kit

[0061] Detection kit (30 persons):

[0062]

[0063] 2. How to use the HSP60 detection kit

[0064] The method of use is as follows:

[0065] 1. Sample dialysis

[0066] Extract blood from lung cancer patients and healthy controls, centrifuge after EDTA anticoagulation (1200rpm, 10min), absorb the supernatant and perform a second centrifugation (3000rpm, 15min), absorb the supernatant after the second centrifugation, namely plasma, as a test Specimens were stored at -80°C after aliquoting.

[0067] Plasma samples were dialyzed using dialysis tubing prior to biotin labeling. 100 μL of samples were taken respectively and added into dialysis tubes, and then dialyzed in 4000 mL of 1×PBS (pH=8) at 4° C. while stirring. The dialysate was changed every 3 hours, and the sam...

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Abstract

The invention discloses a lung cancer screening kit which comprises an optional reagent used for detecting the HSP60 expression level. The invention further discloses application of the reagent for detecting the HSP60 expression level in preparing a reagent for screening lung cancer. The risk of lung cancer of crowds to be examined can be judged when the kit is used for detecting the HSP60 expression level, the kit can be used for clinic lung cancer auxiliary diagnosis, effective basis is provided for patients for adopting relevant treatment measures or decisions, and the clinic application prospect is good.

Description

technical field [0001] The invention relates to a lung cancer screening kit. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] The etiology of lung cancer is complex, and it is generally believed that the influencing factors include: ① smoking; ② environmental pollution: such as smog, interior decoration; ③ unhealthy lifestyle: such as poor eating habits, high life pressure; Silicosis, chronic bronchitis; ⑤ Intrinsic factors in the human body: such as family inheritance, decreased immune function, endocrine dysfunction, etc. [0004] At the same time, lung cancer is a disease that is good at hiding. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 张立李为民刘伦旭刘丹王业唐怀蓉黄燕田攀文朱苡晗谭政堂王彦文
Owner WEST CHINA HOSPITAL SICHUAN UNIV