Preparation method of composite strain fermented cottonseed meal

A technology of compound strains and cottonseed meal, which is applied in application, animal feed, animal feed, etc., can solve the problems of high nutritional value, poor palatability, and failure to meet safe feeding standards, and achieve simple fermentation process, improved utilization rate, The effect of improving palatability

Inactive Publication Date: 2016-06-22
TIANJIN JIARUIXING TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that many microorganisms can degrade free gossypol, but the cotton meal feed produced by fermenting cotton meal with a single strain of bacteria, some achieve detoxification effect but poor palatability, and some have high nutritional value but fail to meet the safe feeding standard

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing cottonseed meal fermented by composite strains, comprising the steps of:

[0023] The first step, the activation of the strain;

[0024] Inoculate Bacillus subtilis into beef extract peptone medium, culture at 35°C, 110r / min for 15h, activate the strain, and obtain the first-grade strain of Bacillus subtilis;

[0025] Inoculate Candida aeruginosa into PDA medium, culture at 24° C. and 110 r / min for 15 hours, activate the strain, and obtain the first-class strain of Candida aeruginosa;

[0026] The second step is the preparation of seeds for production;

[0027] Inoculate the primary strain of Bacillus subtilis obtained in the first step into the beef extract peptone medium, and cultivate it at 35° C. at 110 r / min for 20 hours to obtain the secondary strain of Bacillus subtilis;

[0028] Inoculate the primary strain of Candida utilis obtained in the first step into the PDA medium, and cultivate it at 24° C. at 110 r / min for 24 hours to obtain the...

Embodiment 2

[0038] A method for preparing cottonseed meal fermented by composite strains, comprising the steps of:

[0039] The first step, the activation of the strain;

[0040] Inoculate Bacillus subtilis into beef extract peptone medium, culture at 30°C, 120r / min for 18h, activate the strain, and obtain the first-grade strain of Bacillus subtilis;

[0041] Inoculate Candida aeruginosa into PDA medium, culture at 28°C and 120r / min for 18 hours, and activate the strain to obtain the first-class strain of Candida aeruginosa;

[0042] The second step is the preparation of seeds for production;

[0043] Inoculate the primary strain of Bacillus subtilis obtained in the first step into the beef extract peptone medium, and cultivate it at 30° C. at 120 r / min for 12 hours to obtain the secondary strain of Bacillus subtilis;

[0044] Inoculate the first-grade strain of Candida utilis obtained in the first step into the PDA medium, and cultivate it at 28° C. at 120 r / min for 24 hours to obtain ...

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PUM

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Abstract

The invention belongs to the technical field of microbial fermentation, and concretely relates to a preparation method of composite strain fermented cottonseed meal. The method is characterized in that Bacillus subtilis and Candida utilis are adopted as composite active strains, solid fermentation is adopted, and fermented cottonseed meal is dried, crushed, examined and packaged. A fermentation technology is simple, effectively degrades toxic substances in the cottonseed meal, and improves the protein quality of the cottonseed meal, so the fermented cottonseed meal obtained in the invention has the advantages of good palatability, promotion of digestion and absorption of nutrients by animals, and improvement of the animal production, thereby the utilization rate of the cottonseed meal in various livestock and poultry feeds is increased.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for preparing cotton meal fermented by composite bacteria. Background technique [0002] Cottonseed meal is the residue after oil extraction of cottonseed, and its crude protein content is 36% to 41%, which is a rich protein resource. my country is a big cotton producing country in the world, with an annual production of about 600,000 tons of cotton meal, of which less than 30% is effectively utilized. Cotton meal contains toxic substances such as free gossypol, high crude fiber content and poor palatability, which limits its utilization in the feed industry. In recent years, with the development of animal husbandry, my country's protein feed resources are seriously lacking, so the development and utilization of cotton meal is particularly important. Studies have shown that many microorganisms can degrade free gossypol, but cotton meal feed p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14
Inventor 周勇
Owner TIANJIN JIARUIXING TECH DEV
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