Preparation method of composite strain fermented cottonseed meal
A technology of compound strains and cottonseed meal, which is applied in application, animal feed, animal feed, etc., can solve the problems of high nutritional value, poor palatability, and failure to meet safe feeding standards, and achieve simple fermentation process, improved utilization rate, The effect of improving palatability
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Embodiment 1
[0022] A method for preparing cottonseed meal fermented by composite strains, comprising the steps of:
[0023] The first step, the activation of the strain;
[0024] Inoculate Bacillus subtilis into beef extract peptone medium, culture at 35°C, 110r / min for 15h, activate the strain, and obtain the first-grade strain of Bacillus subtilis;
[0025] Inoculate Candida aeruginosa into PDA medium, culture at 24° C. and 110 r / min for 15 hours, activate the strain, and obtain the first-class strain of Candida aeruginosa;
[0026] The second step is the preparation of seeds for production;
[0027] Inoculate the primary strain of Bacillus subtilis obtained in the first step into the beef extract peptone medium, and cultivate it at 35° C. at 110 r / min for 20 hours to obtain the secondary strain of Bacillus subtilis;
[0028] Inoculate the primary strain of Candida utilis obtained in the first step into the PDA medium, and cultivate it at 24° C. at 110 r / min for 24 hours to obtain the...
Embodiment 2
[0038] A method for preparing cottonseed meal fermented by composite strains, comprising the steps of:
[0039] The first step, the activation of the strain;
[0040] Inoculate Bacillus subtilis into beef extract peptone medium, culture at 30°C, 120r / min for 18h, activate the strain, and obtain the first-grade strain of Bacillus subtilis;
[0041] Inoculate Candida aeruginosa into PDA medium, culture at 28°C and 120r / min for 18 hours, and activate the strain to obtain the first-class strain of Candida aeruginosa;
[0042] The second step is the preparation of seeds for production;
[0043] Inoculate the primary strain of Bacillus subtilis obtained in the first step into the beef extract peptone medium, and cultivate it at 30° C. at 120 r / min for 12 hours to obtain the secondary strain of Bacillus subtilis;
[0044] Inoculate the first-grade strain of Candida utilis obtained in the first step into the PDA medium, and cultivate it at 28° C. at 120 r / min for 24 hours to obtain ...
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