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Preparation method of vegetable seed protein feed

A rapeseed protein and feed technology, applied in the direction of animal feed, animal feed, additional food elements, etc., can solve the problems of insufficient utilization of microbial resources, damage to the original configuration of amino acids, unfavorable animal feed production, etc., and achieve environmental benefits. Protection, promotion of digestion and absorption, growth and development, low-cost effect

Inactive Publication Date: 2012-04-25
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acid or alkali added in the production of protein feed by the chemical detoxification method will hydrolyze the protein in the rapeseed meal and produce chloropropanol carcinogens, and the reaction conditions are severe, destroying the original configuration of the amino acid, and at the same time hydrolyzing the waste liquid Emissions will also cause certain environmental pollution, which is not conducive to the production of animal feed
Microbial fermentation detoxification to produce feed protein, the process is simple, and the amount of feed added is large, which can be used for large-scale production of rapeseed feed protein. role, can not fully utilize microbial resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Screening of strains of solid-state fermented rapeseed meal to produce rapeseed protein feed

[0018] Screening objects: Aspergillus niger, Geotrichum candidum, Aspergillus oryzae, Aspergillus usami, Actinomucor elegans, lactic acid bacteria, Candida utilis, Bacillus licheniformis and Bacillus subtilis.

[0019] Preparation of starter: after the expanded culture of the activated bacterial classification (cultivated by a conventional method), dilute the fermented seeds with sterile water to make the concentration of thalline or spores reach 3×10 7 Each / mL is the starter.

[0020] The double-low rapeseed meal was crushed to a particle size of about 500 μm, and sterilized by moist heat at 121°C for 30 minutes. In the fermenter, the starter is added according to the solid-liquid ratio of 1:1-1:2 (mass ratio), the fermentation temperature is 28°C-35°C, the ambient humidity is 80%-90%, stirring and ventilating. After 3 days of fermentation, distilled water was add...

Embodiment 2

[0022] Example 2 Production of rapeseed protein feed by solid-state fermentation of rapeseed meal mixed with Bacillus subtilis and Mucor radiata

[0023] Preparation of Bacillus subtilis suspension:

[0024] Slant medium: 3g beef extract, 10g peptone, NaCl5, 20g agar, 1000mL tap water, pH 7.2-7.4.

[0025] Seed medium: beef extract 3g, peptone 10, NaCl 5g, tap water 1000mL, pH 7.2-7.4.

[0026] Nutrient solution composition: glucose 2.6g, KH 2 PO 4 2.6g, sterile water 1000mL, pH 7.0.

[0027] Pick two rings of Bacillus subtilis from the activated strain preservation slant and inoculate it in the seed medium, seal it with 8 layers of gauze, culture it on a shaker at 35°C, 120r / min for 24 hours, and the concentration of bacteria reaches 10 8 individual / mL.

[0028] Dilute the fermented liquid with sterile nutrient solution to prepare the cell concentration of 3×10 7 Individual / mL suspension of Bacillus subtilis.

[0029] Preparation of Mucor actinosa suspension:

[0030]...

Embodiment 3

[0037] Example 3 Production of rapeseed protein feed by solid-state fermentation of rapeseed meal with Mucor radiata and Candida utilis synchronously mixed

[0038] Preparation of Mucor actinosa suspension:

[0039]Slant medium: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0040] Plate expansion medium: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0041] Sterile nutrient solution: glucose 5g, KH 2 PO 4 2g, 1000mL sterile water, pH 6.5.

[0042] Mucor actinosa was inoculated on a slant medium and cultured at a constant temperature of 28°C for 4 days, and then inoculated on a flat plate for expansion culture. After 4 days, the spores were washed with sterile nutrient solution, and the concentration was adjusted to 3×10 7 individual / mL.

[0043] Preparation of Candida utilis suspension:

[0044] Slant medium: 3g of malt extract, 10g of glucose, 3g of yeast extract, 5g of peptone; 20g of agar, 1000mL of tap water.

[0045] Seed medium: malt extract 3g,...

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PUM

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Abstract

The invention provides rapeseed protein feed which is solid powder or particulate matter. The content of protein is between 35 and 50 percent (N * 6.25, dry basis); the content of water-soluble nitrogen is between 25 and 35 percent; the content of small peptide is between 10 and 25 percent; the content is counted by weight percentage; and thioglycoside accounts for 5 to 15 micromole per gram of rapeseed protein feed. The invention also provides a method for preparing the rapeseed protein feed; one of bacillus subtilis, lactobacillus or candida utilis and actinomucor elegans or aspergillus usamii are compounded to prepare a fermenting agent; the fermenting agent is added to pulverized rapeseed dreg for solid fermentation; and a culturing substrate is sterilized, dried and pulverized to obtain the rapeseed protein feed rich in small peptide. The preparation method is environment-friendly, has mild reaction condition, can fully exert the functions of protease hydrolysis and thioglycosidedegradation of microbe and is a method which has low cost and is suitable for the industrialized large-batch preparation of the rapeseed protein feed.

Description

technical field [0001] The invention relates to a feed, in particular to a rapeseed protein feed obtained by using rapeseed meal as a raw material, and also relates to a preparation method of the vegetable protein feed-a mixed-bacteria solid-state fermentation method. Background technique [0002] Rapeseed is an oil crop produced in a large area in my country, and its output ranks first in the world. Rapeseed oil is also the main edible vegetable oil for Chinese residents. The by-products produced in the oil processing process are huge. The output of rapeseed meal alone exceeds 1,000 per year. million t. As the main by-product of the oil processing industry, rapeseed meal contains 35% to 50% of crude protein, and the content of lysine, cystine and methionine in rapeseed protein is relatively high, and the amino acid balance is better than that of soybean protein. It is a high-quality plant protein source, but due to the presence of glucosinolates and some anti-nutritional fa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16A23K1/14A23K10/12A23K20/147
Inventor 袁建鞠兴荣何荣王立峰顾建洪
Owner NANJING UNIV OF FINANCE & ECONOMICS
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