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Method for preparing vegetable seed active peptide by microbial solid state fermentation

A solid-state fermentation and active peptide technology, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and peptide preparation methods, can solve the problems of destroying the original configuration of amino acids, severe reaction conditions, and low degree of hydrolysis. It is beneficial to environmental protection, the production process is simple, and the effect of wide source of raw materials

Inactive Publication Date: 2012-10-10
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the chemical method uses acid or alkali to hydrolyze vegetable protein, chloropropanol carcinogens will be produced, and the reaction conditions are severe, destroying the original configuration of amino acids, and the discharge of hydrolysis waste liquid will also cause certain environmental pollution
Enzymatic hydrolysis is currently the main method for the preparation of rapeseed peptides, but since the anti-nutritional factors in rapeseed meal or rapeseed protein isolate cannot be completely removed, the activity of protease is affected, the degree of hydrolysis is not high, and the flavor of rapeseed protein hydrolyzate In particular, the problem of bitterness has not been fundamentally resolved, and the source of enzymes used for hydrolysis of rapeseed protein is limited, and the cost remains high, so the enzymatic preparation of rapeseed active peptides is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Screening of solid-state fermented rapeseed meal to produce rapeseed active peptide strains

[0020] Fermentation strains: Aspergillus niger, Rhizopus niger, Geotrichum candidum, Aspergillus oryzae, Aspergillus usami, Mucor actinosa, Lactic acid bacteria, Candida utilis, Bacillus licheniformis and Bacillus subtilis.

[0021] Preparation of starter: After the activated strains were expanded and cultivated according to conventional methods, the fermented seeds were diluted with sterile distilled water to make the cell concentration reach 3×10 7 Each / mL is the starter.

[0022] The double-low rapeseed meal was crushed to a particle size of about 500 μm, and sterilized by moist heat at 121°C for 30 minutes. In the fermenter, the starter is added according to the solid-liquid ratio of 1:1-1:2 (mass ratio), the fermentation temperature is 28°C-35°C, the ambient humidity is 80%-90%, stirring and ventilating. After 3 days of fermentation, add distilled water to diss...

Embodiment 4

[0024] Example 4 Rapeseed Active Peptide Production by Aspergillus Usami Solid-state Fermentation of Rapeseed Meal

[0025] Slant medium: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0026] Plate expansion medium: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0027] Nutrient solution composition: glucose 50g, KH 2 PO 4 2g, tap water 1000mL, pH 6.0.

[0028] Fermentation medium: rapeseed meal, nutrient solution.

[0029] Preparation of starter: Aspergillus Usami was inoculated on a slant medium and cultured at a constant temperature of 28°C for 4 days, then inoculated on a flat plate and expanded at 28°C, after 4 days, the spores were washed with sterile nutrient solution, and the concentration was adjusted to 10 7 individual / mL.

[0030] Crush the common rapeseed meal to a particle size of about 400 μm, sterilize, add a starter in a fermenter at a solid-to-liquid ratio of 1:1 (mass ratio), adjust the fermentation temperature to 30°C, and the ambient h...

Embodiment 5

[0032] Example 5 Candida utilis solid-state fermentation of rapeseed meal to produce rapeseed active peptide

[0033] Incline medium: 3g of malt extract, 10g of glucose, 3g of yeast extract, 5g of peptone, 20g of agar, 1000mL of tap water.

[0034] Seed medium: malt extract 3g, glucose 10g, yeast extract 3g, peptone 5g, tap water 1000mL.

[0035] Nutrient solution: glucose 3g, KH 2 PO 4 3g, tap water 1000mL, pH 7.0.

[0036] Fermentation medium: rapeseed meal, nutrient solution.

[0037] Preparation of starter: Pick two rings of Candida utilis from the slant of preserved strains after activation and inoculate it in the seed medium, seal with 8 layers of gauze, culture at 35°C, 120r / min, on a shaking table for 24h, the bacteria Concentration up to 10 8 individual / mL. Adjust its concentration to 10 with sterile nutrient solution 7 pcs / mL

[0038] Crush the rapeseed meal to a particle size of about 300 μm, and sterilize at 121°C for 30 minutes. In the fermenter, add the ...

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Abstract

The invention provides a method for preparing rapeseed bioactive peptide by micro-organism solid-state fermentation, comprising the steps: rapeseed meal obtained from pressing or leaching oil making process is crushed for obtaining crushed rapeseed meal; single species with protease production capacity such as Bacillus subtilis, lactic acid bacteria, Actinomucor elegans, usamii Aspergillus or Candida yeast is used for preparing hametz; the hametz is introduced into the crushed rapeseed meal for conducting solid-state fermentation; water is added for extracting medium after solid-state fermentation and then residue is removed by separation for obtaining the crude extract of the rapeseed bioactive peptide; and finally, the crude extract is decolored, desalted and dried for obtaining the rapeseed bioactive peptide. The method is environment-friendly, has mild reaction conditions, can fully play the role of the protease hydrolysis and glucosinolate degradation of micro-organisms, and is applicable to batch preparation of rapeseed bioactive peptide with low cost.

Description

technical field [0001] The invention relates to a method for preparing rapeseed active peptide, in particular to a method for preparing rapeseed active peptide by solid-state fermentation and hydrolyzing rapeseed dregs with microorganisms capable of producing protease. Background technique [0002] Rapeseed meal is a by-product of rapeseed oil production, which is rich in protein and is an important plant protein resource. Because rapeseed meal contains anti-nutritional components such as glucosinolates and phytic acid, rapeseed meal is used as a plant protein The application of resources in the food industry is limited. Rapeseed protein is mainly composed of 12S globulin and 2S or 1.7S albumin. The content of lysine, cystine and methionine is relatively high. The amino acid balance is better than that of soybean protein. It is a high-quality protein. Rapeseed protein is hydrolyzed into polypeptides Finally, it has better biological activity and processing characteristics. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/18C12R1/72C12R1/66C12R1/785C12R1/225C12R1/125
Inventor 王立峰鞠兴荣袁建何荣
Owner NANJING UNIV OF FINANCE & ECONOMICS
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