Agrocybe aegirit water-soluble polysaccharide separation and purification technology

A water-soluble polysaccharide, separation and purification technology, which is applied in the field of separation and purification of tea tree mushroom water-soluble polysaccharide, and can solve problems such as no detailed reports.

Inactive Publication Date: 2016-07-06
ZHENGZHOU GUOSHOU BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no detailed report on the identification of tea tree mushroom water-soluble polysaccharide, one of the main functional components in tea tree mushroom

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0005] Example: a. Extraction of tea tree mushroom water-soluble polysaccharide: crush the tea tree mushroom raw material into fine particles that can pass through a 30-mesh sieve, reflux the ethanol, add 4 times the amount of 95% ethanol to mix, and reflux for 3 hours; extraction, based on previous work Basis, extraction conditions: extraction temperature 80℃, time 1.5h, solid-liquid ratio 1:25; centrifugation, 3000r / min, centrifugation 15min; vacuum concentration, concentrated to 1 / 4 of the original volume; alcohol precipitation, 4 times Precipitate with 95% ethanol; to remove protein, add chloroform equivalent to 1 / 4 volume of polysaccharide aqueous solution, then add n-butanol with 1 / 16 volume of chloroform, shake vigorously for 10 minutes, and centrifuge to remove denatured protein at the interface between the water layer and the solvent ; Repeat the operation several times until no denatured protein appears; dialysis, running water dialysis for 48 hours, distilled water d...

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PUM

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Abstract

The present invention discloses an agrocybe aegirit water-soluble polysaccharide separation and purification technology. The technology method is as follows: the study provides a theoretical basis for the research and development of agrocybe aegirit health-care food, studies a functional factor in the agrocybe aegirit-fungus water-soluble polysaccharides, firstly conducts separation and purification in the study to obtain five agrocybe aegirit water-soluble polysaccharides, and further analyses and studies the physical and chemical composition of polysaccharide AP2 with a maximum content. The result shows that the AP2 molecular weight is 122,460, the AP2 is composed of acidic polysaccharides of D-glucose, D-mannose, D-xylose and galacturonic acid, wherein the galacturonic acid content is 6.8%.

Description

technical field [0001] The invention relates to a separation and purification process of tea tree mushroom water-soluble polysaccharide. Background technique [0002] Edible fungi are a large class of food resources with high nutritional and medicinal value. Since Chihara, Japan reported the isolation of an anti-tumor polysaccharide from shiitake mushrooms in 1970, which caused a sensation in the entire medical field, there has been a wave of research all over the world combining the research of edible fungi with polysaccharides to find new anti-tumor drug resources from edible fungi. upsurge. Studies have shown that water-soluble polysaccharides in edible fungi have immunoregulatory effects, mainly by activating the immune system. The high and low anti-tumor activity is related to the spatial structure of polysaccharide molecules. Factors that affect the spatial structure, such as side chains and molecular weights, affect the inhibitory effect tumor. In recent years, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 冯松平
Owner ZHENGZHOU GUOSHOU BIOLOGICAL TECH CO LTD
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