Tumor marker and application thereof

A tumor marker and tumor technology, applied in tumor markers and their application fields, can solve the problem of lack of gene function, and achieve a highly specific effect

Active Publication Date: 2016-07-13
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The matrix remodeling-associated 7 (MXRA7) gene was first discovered in bioinformatics analysis and named according to the genes associated with its expression, but no studies on the function of this gene have been performed so far

Method used

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  • Tumor marker and application thereof
  • Tumor marker and application thereof
  • Tumor marker and application thereof

Examples

Experimental program
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Embodiment 1

[0030] The GEO database has many publicly published gene chip data around the world, and the GDS data is a single-sample experimental data set on a certain topic that is manually compiled. The present invention searches the GEO database for possible functions of MXRA7. Search for "MXRA7" in "GEOProfiles" in Pubmed, and the returned results are 5829. The cut-off time is August 27, 2015. When the results are displayed as "subgroup effect", 3 of the first 5 results are related to cancer tissue or cancer cell related. First, the estrogen receptor α was knocked out in the breast cancer cell line (MCF7), and the results of GDS4061 showed that the expression of MXRA7 increased by nearly 50 times, because the estrogen receptor plays an important role in determining the behavior of breast cancer cells, so the study Differential expression of MXRA7 in patients with such diseases may be of great value. GDS1667 data showing correlation of MXRA7 expression levels with human papillomaviru...

Embodiment 2

[0033]Oncomine database, a cancer microarray database and web-based data mining platform designed to facilitate discoveries from genome-wide expression analysis, the present invention explores the link between MXRA7 and tumor biology by analyzing the database. By varying the search options such as different multiples (1.5, 2, 3, 4, 6, 8, 10, 20), domain-specific P values ​​(5×10 -2 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 ), gene expression levels (top 1%, 5%, 10%, or all) data are available for varying degrees of involvement of MXRA7. As of August 27, 2015, Oncomine contains 715 data sets, including a total of 86733 samples. By setting corresponding search conditions (1.5 or 2.0 fold change, P value Figure 4 shown. In breast cancer, MXRA7 was down-regulated by two times in 13 samples, and MXRA7 expression level ranked in the top 10% in 11 samples. Also in prostate cancer, MXRA7 was significantly downregulated. However, in esophageal cancer, leukemia and lym...

Embodiment 3

[0035] Real-time quantitative PCR analysis to detect the expression of different transcripts of MXRA7 in different tissues and cancers

[0036] In order to further confirm the relationship between MXRA7 and human cancer in the public database, the present invention compared the expression levels of three transcripts of human MXRA7 gene between cancer tissue and normal tissue by real-time quantitative PCR. The sequences of the three transcripts were obtained from NCBI, namely NR_130926, NR_130927 and NR_130928. The present invention uses TissueScancDNAarray (Cat#CSRT103, Origene, Rockville, MD) to detect expression differences. The data set includes 22 different tissues and 381 human cDNA samples, all of which have complete clinicopathological information. In this example, three pairs of primers and corresponding probes were designed, and multiplex PCR was used to detect the expression of different MXRA7 transcripts in each sample. The real-time quantitative PCR instrument mo...

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Abstract

The invention discloses a tumor marker and an application thereof. The tumor marker comprises a spliceosome 1 or an amino acid sequence SEQ ID NO.1 thereof, a spliceosome 2 or an amino acid sequence SEQ ID NO.2, and a spliceosome 3 or an amino acid sequence SEQ ID NO.3. (1) Three different spliceosomes of MXRA 7 show different change patterns in different tumors, the gene can be expressed in different tumors, and the MXRA 7 has different expression patterns in different stages of the tumors or during the growth and metastasis of the tumors, so that the occurrence, growth, metastasis and the like of different tumors can be determined according to the transcription or expression level of the MXRA7; (2) the MXRA 7 can be used as a target spot of tumor treatment, and a new tumor treatment strategy is provided by regulating and controlling the expression process of the MXRA 7; and (3) the marker disclosed by the invention is strong in specificity, and is suitable for multiple types of tumors.

Description

technical field [0001] The invention belongs to the field of tumor diagnosis and treatment, and relates to the diagnosis and screening of tumors in high-risk groups, the prognosis judgment of tumor patients, the determination of the existence, staging and / or metastasis of tumors, and tumor treatment, and specifically relates to a tumor marker and its application. Background technique [0002] In the field of tumor biology, one of the main research focuses is to find and identify genes and related pathways and molecular mechanisms that are closely related to tumorigenesis, development, metastasis, treatment response, and prognosis, in order to find possible therapeutic effects on tumors. Targets for diagnosis, prognosis, and therapy. Among them, the identification and application of tumor markers are the most representative. Currently recognized tumor markers include tissue-specific markers, such as estrogen receptor ER (breast cancer), prostate-specific antigen PSA (prosta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12Q1/68G01N33/68G01N33/574
CPCC07K14/47C12Q1/6886C12Q2600/106C12Q2600/112C12Q2600/118G01N33/57484G01N33/68G01N2333/47
Inventor 王宜强沈莹胡雅彬宁金玲吴坤王婷
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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