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Ganoderma lucidum strain cultivation method

A technology of culture and Ganoderma lucidum, applied in the field of agriculture, can solve the problems of slow growth of mycelium, low rate of mushroom formation and poor resistance, etc., and achieve the effects of fast growth of mycelium, high rate of mushroom formation and strong resistance.

Active Publication Date: 2016-08-03
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

[0006] In production, the purification and rejuvenation of Ganoderma lucidum strains are generally carried out by separating the young edge part of the sporocarp and the primordium for purification and culture. Although this method is easy to operate, the mycelia of the isolated strains grow slowly. It takes 10-12 days to grow fully; and the resistance is poor, and the rate of mushroom formation is low

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  • Ganoderma lucidum strain cultivation method
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  • Ganoderma lucidum strain cultivation method

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Embodiment Construction

[0026] Below in conjunction with example the present invention is described in further detail.

[0027] A method for cultivating red sesame strains, said method comprising the steps of:

[0028] 1. In the red sesame cultivation shed, select a single-flowered mushroom culture bag with typical fruiting bodies, pure color, correct mushroom shape, and no pests and diseases. The mushroom bag comes from the production base of Jiangsu Edible Fungi Research Institute, and the strain is Chizhi No. 1.

[0029] 2. Wipe the surface of the cultivation bag with 3-5% potassium permanganate solution;

[0030] 3. Move the cultivation bag into the inoculation box or ultra-clean workbench, and use a sterilized scalpel or single blade to remove the 0.5cm square bag film in the middle and lower part of the bag;

[0031] 4. Use a sterilized inoculation needle to remove the old hyphae on the surface, pick a medium with mycelium the size of rice grains in the culture material, and connect it to the...

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Abstract

The invention discloses a ganoderma lucidum strain cultivation method which comprises the following steps: (1) selecting a single-ganoderma lucidum fungus sack with a typical sporocarp, a pure color, good shape and no pest and disease damage; (2) wiping the cultivation sack with a disinfectant; (3) cutting off a sack film of 0.5cm<3> at the middle lower part of the cultivation sack with a blade; (4) picking hypha in a cultivation medium with an inoculating needle through an incision formed in the step (3), and inoculating the hypha into test tubes; (5) cultivating the test tubes obtained in the step (4) under a shading condition of the temperature of 25 to 28 DEG C for 2 to 3 days, selecting the test tube with robust hypha, picking newly produced hypha at the edge, transferring the hypha into a new culture medium for cultivation, to obtain pure hypha bodies which are strains for reproducing ganoderma lucidum; (6) manufacturing reproduction mother strains and stock strains from the strains obtained in the step (5) according to a conventional strain production procedure and method. According to the ganoderma lucidum strain cultivation method, the hypha obtained by separation grows quickly, and is high in resistance, high in mushroom yield and high in yielding ability.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for cultivating red sesame strains. Background technique [0002] Ganoderma Lucidum Karst (Ganoderma Lucidum Karst) is umbrella-shaped in shape, with a kidney-shaped, semicircular or nearly round cap. Ganoderma lucidum has been used medicinally in my country for more than 2,000 years, and it has been regarded as a magical treasure for nourishing and strengthening, strengthening the body and strengthening the foundation. It has the effects of invigorating qi and calming the nerves, relieving cough and relieving asthma, and is used for dizziness, insomnia, palpitation, shortness of breath, cough and asthma due to fatigue. [0003] At present, there are more than 100 species of fungi in the genus Ganoderma, among which G.lu-cidum is the most widely distributed, followed by G.japonicum, and G.applana-tum, Both G.tsugae and G.capense are used for medicine. ...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 谢春芹曹正凡军民谢正林
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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