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A kind of artificial reproduction method of white moth and black stick larvae

A technology of black stick biting wasps and artificial reproduction, which is applied in the field of insect reproduction to achieve the effects of increasing the number of bees, increasing the parasitism rate, and reducing costs

Active Publication Date: 2018-10-26
LUOHE CITY FOREST PEST CONTROL & QUARANTINE STATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no literature reports on the use of tussah silkworm chrysalis and other records to reproduce the white moth black gnat on a large scale.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: a kind of artificial breeding method of white moth black stick gnat, comprises the following steps:

[0031] (a) Select an alternative host: use the autumn chrysalis of Northeast tussah silkworm as an alternative host and store them in a cold storage with the temperature controlled at 4°C and the relative humidity at 70%; Cocoon, gradually warming up to ambient temperature for standby after 2d;

[0032] (b) Field collection and screening of Yang Xiaozhou moth pupae: collect Yang Xiaozhou moth pupae in nature, and screen parasitic pupae and Yang Xiaozhou moth parasitic pupa for standby; Yang Xiaozhou moth parasitic pupa body surface color darkens, The internodes of the pupal body are elongated, and the surface of the unparasitized pupae of Yang Xiaozhou moth is bright;

[0033] (c) kind of bee cultivation: the parasitic pupa of Yang Xiaozhou moth is put into the test tube for cultivation, and after eclosion, it is used as the kind of bee of white moth blac...

Embodiment 2

[0038] Embodiment 2: a kind of artificial propagation method of white moth black stick gnat, comprises the following steps:

[0039] (a) Select an alternative host: use the autumn chrysalis of Northeast tussah silkworm as an alternative host and store them in a cold storage with the temperature controlled at 6°C and the relative humidity at 80%; Cocoon, gradually warming up to ambient temperature for standby after 2d;

[0040] (b) Field collection and screening of Yang Xiaozhou moth pupae: collect Yang Xiaozhou moth pupae in nature, and screen parasitic pupae and Yang Xiaozhou moth parasitic pupa for standby; Yang Xiaozhou moth parasitic pupa body surface color darkens, The internodes of the pupal body are elongated, and the surface of the unparasitized pupae of Yang Xiaozhou moth is bright;

[0041] (c) kind of bee cultivation: the parasitic pupa of Yang Xiaozhou moth is put into the test tube for cultivation, and after eclosion, it is used as the kind of bee of white moth b...

Embodiment 3

[0046] Embodiment 3: a kind of artificial breeding method of white moth black stick gnat, comprises the following steps:

[0047](a) Select an alternative host: use the autumn chrysalis of Northeast tussah silkworm as an alternative host, and store them in a cold storage with a temperature controlled at 5°C and a relative humidity of 75%; Cocoon, gradually warming up to ambient temperature for standby after 2d;

[0048] (b) Field collection and screening of Yang Xiaozhou moth pupae: collect Yang Xiaozhou moth pupae in nature, and screen parasitic pupae and Yang Xiaozhou moth parasitic pupa for standby; Yang Xiaozhou moth parasitic pupa body surface color darkens, The internodes of the pupal body are elongated, and the surface of the unparasitized pupae of Yang Xiaozhou moth is bright;

[0049] (c) kind of bee cultivation: the parasitic pupa of Yang Xiaozhou moth is put into the test tube for cultivation, and after eclosion, it is used as the kind of bee of white moth black st...

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Abstract

The invention discloses a method for artificial propagation of the black-crowned wasp, which comprises the following steps: (a) selecting an alternative host, (b) collecting and screening the pupae of the moth moth in the field, (c) cultivating bee species, (d) Information material extraction, (e) tussah silkworm chrysalis treatment, (f) inoculation with bees, (g) cultivation and development. The present invention obtains the pupal surface extract by soaking the unparasitic pupa in alcohol, and then sprays it on the tussah chrysalis by the method of artificial spraying, induces the parasitism of the black rod gnat, and improves the parasitic rate; adopts indoor large-scale propagation of the black rod gnat, for Forest biological control of Lepidopteran pests such as American white moth and poplar moth provides practical technical means.

Description

technical field [0001] The invention relates to the field of insect propagation, in particular to an artificial propagation method of the black-cripped wasp. Background technique [0002] The white moth Tetrastichus septentrionalis Yang is an important parasitic natural enemy in the pupal stage of various Lepidoptera leaf-eating pests such as American white moth, Yang Xiaozhou moth, and Yang fanzhou moth. It is widely distributed in northern my country. A relatively common natural enemy insect in the pupal stage of the order pests. Generally, 86-349 bees can emerge from each pupa of the American white moth, with an average of 160 bees. The wasp adult is relatively large, strong in body, strong in flying ability, and strong in vitality. It can survive for more than 15 days at room temperature. The natural parasitism rate in the pupae of the American white moth is sometimes as high as 12% to 24%. It has great potential in biological control. Good use prospects. [0003] Tussa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 郭振业刘全山郭俊强孙会灵陈慧杰闫俊香承青春张合成任慧娟杨金泽盛文李洪雨薛科郭小伟刘白璐韩培万燕华李亚杰刘方方李国强张峰葛瑞琼翟耀东李大银白纪霞林艳平万陈真王炳焱姬锟
Owner LUOHE CITY FOREST PEST CONTROL & QUARANTINE STATION
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