Chlorpropamide pharmaceutical composition and medical application thereof

Abstract

The invention discloses a pharmaceutical composition of chlorpropamide and its medical application. The pharmaceutical composition of chlorpropamide provided by the invention contains chlorpropamide and a kind of The novel natural product compound (Ⅰ), chlorpropamide, compound (Ⅰ) can protect the activity of antioxidant enzymes in rats, scavenge free radicals, and inhibit lipid peroxidation, suggesting that antioxidants are chlorpropamide, One of the mechanisms by which compound (I) protects the kidneys of DM rats. After the combined use of chlorpropamide and compound (I), the blood lipid regulation effect and renal protection effect on rats with type 2 diabetes were further improved. Early application can effectively delay the occurrence and development of diabetic nephropathy, and can be developed into a treatment for diabetic nephropathy. The drug for pathological changes has outstanding substantive features and significant progress compared with the prior art.

Description

technical field

[0001] The invention belongs to the field of biomedicine and relates to a new application of chlorpropamide, in particular to a pharmaceutical composition of chlorpropamide and its medical application. Background technique

[0002] The indication of chlorpropamide is suitable for mild and moderate type Ⅱ diabetes mellitus whose curative effect is not satisfied with diet alone. The patient's islet β cells have a certain function of secreting insulin, and there is no serious complication. This product can also be used for central diabetes insipidus.

[0003] So far, there is no report on the correlation between chlorpropamide and its pharmaceutical composition and diabetic cardiomyopathy. Contents of the invention

[0004] The object of the present invention is to provide a pharmaceutical composition of chlorpropamide, which contains chlorpropamide and a natural product, and the chlorpropamide and the natural product can synergistically treat diabetic nephro...

Examples

Embodiment 1

[0020] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0021] Sources of reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane were of analytical grade, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, of analytical grade, were purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd.

[0022] Separation method: (a) Grind the dry root tuber (2kg) of Morinda officinalis, extract with 80% ethanol under heat reflux (15L×3 times), combine the extracts, concentrate until no alcohol smell (3L), and then use petroleum ether ( 3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b ) The n-butanol extract in step (a) is removed with D101 type macroporous resin, first elutes 8 column volumes with 10% ethanol, then elutes 10 column volumes with 70% ethanol, collects 70% eluat...

Embodiment 2

[0025] Embodiment 2: pharmacological action

[0026] 1. Materials and methods

[0027] 1.1 Animals

[0028] 40 Wistar rats aged 3 to 4 months, half male and half male, weighing 210 g to 250 g, were provided by the Experimental Animal Center of Jiamusi University. The animals were kept in a ventilated and dry environment with a temperature of (22±1)°C, a relative humidity of 55%-65%, and a 12-h light cycle, and were fed with maintenance diet 1022 for rats and mice.

[0029] 1.2 Reagents and samples

[0030] Chlorpropamide was purchased from China National Institutes for Food and Drug Control. Compound (I) is self-made, and the preparation method is shown in Example 1. Streptozotocin (STZ) was purchased from Sigma Company of the United States; SOD, Coomassie Brilliant Blue protein assay kits were purchased from Nanjing Jiancheng Bioengineering Institute. Triglyceride kit was purchased from Changchun Huili Engineering Technology Development Center.

[0031] 1.3 Instruments ...

technical field

[0001] The invention belongs to the field of biomedicine and relates to a new application of chlorpropamide, in particular to a pharmaceutical composition of chlorpropamide and its medical application. Background technique

[0002] The indication of chlorpropamide is suitable for mild and moderate type Ⅱ diabetes mellitus whose curative effect is not satisfied with diet alone. The patient's islet β cells have a certain function of secreting insulin, and there is no serious complication. This product can also be used for central diabetes insipidus.

[0003] So far, there is no report on the correlation between chlorpropamide and its pharmaceutical composition and diabetic cardiomyopathy. Contents of the invention

[0004] The object of the present invention is to provide a pharmaceutical composition of chlorpropamide, which contains chlorpropamide and a natural product, and the chlorpropamide and the natural product can synergistically treat diabetic nephro...

Examples

Embodiment 1

[0020] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0021] Sources of reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane were of analytical grade, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, of analytical grade, were purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd.

[0022] Separation method: (a) Grind the dry root tuber (2kg) of Morinda officinalis, extract with 80% ethanol under heat reflux (15L×3 times), combine the extracts, concentrate until no alcohol smell (3L), and then use petroleum ether ( 3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b ) The n-butanol extract in step (a) is removed with D101 type macroporous resin, first elutes 8 column volumes with 10% ethanol, then elutes 10 column volumes with 70% ethanol, collects 70% eluat...

Embodiment 2

[0025] Embodiment 2: pharmacological action

[0026] 1. Materials and methods

[0027] 1.1 Animals

[0028] 40 Wistar rats aged 3 to 4 months, half male and half male, weighing 210 g to 250 g, were provided by the Experimental Animal Center of Jiamusi University. The animals were kept in a ventilated and dry environment with a temperature of (22±1)°C, a relative humidity of 55%-65%, and a 12-h light cycle, and were fed with maintenance diet 1022 for rats and mice.

[0029] 1.2 Reagents and samples

[0030] Chlorpropamide was purchased from China National Institutes for Food and Drug Control. Compound (I) is self-made, and the preparation method is shown in Example 1. Streptozotocin (STZ) was purchased from Sigma Company of the United States; SOD, Coomassie Brilliant Blue protein assay kits were purchased from Nanjing Jiancheng Bioengineering Institute. Triglyceride kit was purchased from Changchun Huili Engineering Technology Development Center.

[0031] 1.3 Instruments ...

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