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Lentinan-modified carbon nano-tube as well as preparation method and application thereof

A technology of lentinan and carbon nanotubes, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, and pharmaceutical formulas, etc. problems, to achieve the effect of improving antibody titer, prolonging drug efficacy, and high grafting rate

Active Publication Date: 2016-08-31
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a carbon nanotube modified with lentinan, so as to solve the problems of unstable lentinan, fast metabolism in the body, low bioavailability and large dosage in the prior art.

Method used

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  • Lentinan-modified carbon nano-tube as well as preparation method and application thereof
  • Lentinan-modified carbon nano-tube as well as preparation method and application thereof
  • Lentinan-modified carbon nano-tube as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Preparation of lentinan carbon nanotubes

[0043] Add 30ml of thionyl chloride and 0.7g of carboxylated carbon nanotubes into the distillation flask, then sonicate for 10min, add 1ml of DMF and sonicate for 3min, reflux at 70°C for 10h (sonication for 10min every 2h), vacuum distillation at 73°C for 1-2h, vacuum at 50°C Dry (ventilate every 3h) until completely dry to obtain acetylated carbon nanotubes, dissolve the acetylated carbon nanotubes with 30ml DMF, add 0.2g lentinan pretreated at 120°C for 2h into DMF, ultrasonicate for 30min, add 1ml Pyridine was sonicated for 3 minutes, and the temperature was controlled at 110°C for 24 hours (sonication for 10 minutes every 3 hours), cooled to room temperature, centrifuged and poured, washed with DMAC / LICL (95 / 5) solution for 5 times, washed twice with PBS, and washed with warm water 5 times , freeze-dried, and stored in a dry place at low temperature.

[0044] Depend on figure 1 The transmission electron mic...

Embodiment 2

[0045] Example 2 Lentinan carbon nanotube immune enhancement activity comparison

[0046] Taking the lentinan carbon nanotubes (L-MWCNTs) prepared in Example 1 as the research object, using lentinan (lentinan) and carboxyl carbon nanotubes (C-MWCNTs) as the contrast, measured their immunity to the model antigen (OVA). The influence of IgG antibody level, antibody subtype, activation of lymph node dendritic cells, OVA antigen and cytokines IFN-γ, TNF-α, IL-12p70, IL-4 and IL-17 in lymph nodes.

[0047] (1) Animal grouping and handling

[0048] Sixty Blab / c mice aged 6-8 weeks were randomly divided into 5 groups, namely lentinan carbon nanotube group (L-MWCNTs), lentinan group (lentinan), carboxyl carbon nanotube group (C-MWCNTs ), the positive control group (FCA) and the PBS control group (PBS). Mice were immunized subcutaneously three times with a one-week interval between each immunization. 7, 14, 21, and 28 days after the first immunization, the IgG antibody levels, antib...

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Abstract

The invention discloses a lentinan-modified carbon nano-tube. The lentinan-modified carbon nano-tube is prepared by grafting a carbon nano-tube with lentinan by virtue of a covalent bond modification method. The invention further discloses a preparation method of the lentinan-modified carbon nano-tube and application of the lentinan-modified carbon nano-tube in preparation of immunological enhancement drugs. Compared with the prior art, the lentinan-modified carbon nano-tube has the advantages that the problems that components of lentinan are easily oxidized and are difficulty prepared into a preparation method are solved, the absorption of drugs is facilitated, and the drugs can be slowly released, so that the drug effect is effectively prolonged, the immunologic functions of animals can be remarkably improved, and the lentinan-modified carbon nano-tube is of an important significance in resisting of diseases. Meanwhile, the lentinan-modified carbon nano-tube is a novel Chinese veterinary drug and has no side effect.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and in particular relates to a carbon nanotube modified by lentinan and its preparation method and application. Background technique [0002] The immunomodulatory activity of lentinan is an important basis for its biological activity. Lentinan is a typical T cell activator, which can promote the production of cytotoxic T lymphocytes (CTL) in vivo and in vitro, increase the killing activity of CTL, and enhance the delayed type hypersensitivity (DTH) of normal or immunocompromised mice. Increases antibody-dependent cytotoxic cell (ADDC) activity. In vivo administration can also induce r-interferon (IFN). Lentinan can also promote T cells to produce macrophage activating factors, which can amplify and activate peritoneal macrophages in vivo, and induce the cytotoxic activity of killer macrophages and antibody-dependent macrophages. Sensitivity of cells. Lentinan can also increase the levels o...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K31/715A61P37/04
CPCA61K31/715
Inventor 王德云邢杰刘振广伯若楠胡元亮刘家国武毅
Owner NANJING AGRICULTURAL UNIVERSITY
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