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Method and culture medium for obtaining lonicera macranthoides regeneration plants through tissue culture

A technique for regenerating plants of Lonicera vulgaris is applied in the field of plant tissue culture, and can solve the problems of not perfecting the regeneration system and hindering the research of Lonicera vulgaris

Inactive Publication Date: 2016-09-07
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no perfect regeneration system for Lonicera cinerea, which hinders further research on Lonicera cinerea, especially the mechanism of its high chlorogenic acid content, multi-flowering and closed flower buds by means of molecular biology. Research

Method used

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  • Method and culture medium for obtaining lonicera macranthoides regeneration plants through tissue culture
  • Method and culture medium for obtaining lonicera macranthoides regeneration plants through tissue culture

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Embodiment 1

[0049] Embodiment 1, obtaining the regenerated plant of Lonicera argentina by tissue culture

[0050] Method I

[0051] 1. Disinfection of explants

[0052] Get gray felted honeysuckle (the public can obtain from Institute of Botany, Chinese Academy of Sciences, this material is quoted from Longhui County, Hunan Province and has been planted in Beijing Botanical Garden, Chinese Academy of Sciences for more than three years; the non-patent literature that has recorded this material is: Tang Xiaorong, Li Wuping, Peng Xiaofeng (2005), Tissue Culture and Rapid Propagation of Lonicera cinerea. Plant Physiology Communications 41(5), 642-642.) New young leaves were used as explants, rinsed with clean water, soaked in running water for 1 hour, and placed in Under aseptic conditions, rinse with sterile water once, disinfect with 70% alcohol for 20 seconds, then disinfect with 2% sodium hypochlorite solution for 4 minutes, then transfer to 1% sodium hypochlorite solution for disinfecti...

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Abstract

The invention discloses a method and culture medium for obtaining lonicera macranthoides regeneration plants through tissue culture. The culture medium for obtaining the lonicera macranthoides regeneration plants is prepared from, by mass, 1,650 parts of major elements (metered by NH4NO3), 0.83 part of trace elements (metered by KI), 37.3 parts of ferric salt (metered by FeSO4.7H2O), 0.5 part of organic ingredients (), 0.1-1.5 parts of zeatin, 0.1-0.5 part of indolebutyric acid and 30,000 parts of saccharose. The differential culture medium for obtaining the lonicera macranthoides regeneration plants is high in pertinence and good in applicability. The method for obtaining the lonicera macranthoides regeneration plants through the tissue culture has the advantages of being not limited by regions, weather and seasons and being beneficial for massively producing single plants with the fine properties.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for obtaining regenerated plants of Lonicera argentina through tissue culture and a culture medium thereof. technical background [0002] Gray wool honeysuckle (Lonicera macranthoides Hand.-Mazz.) is a perennial evergreen twining or erect small shrub plant of the Lonicera family Lonicera. "Chinese Pharmacopoeia" stipulates that Lonicerae cinerea is one of the main plant sources of the medicinal material of mountain silver flower, and its flower buds have a high content of chlorogenic acid, which is a commonly used medicinal part. [0003] A series of varieties formed with Lonicera pilosula as the provenance, with a large number of flower buds, the flower buds are aggregated into umbels or clusters of inflorescences, the corolla is neat, the development period is consistent and the flowering period is concentrated, the flowers are almost not open, and can be pi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 王亮生吴杰苏上
Owner INST OF BOTANY CHINESE ACAD OF SCI
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