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Method for identifying salmon variety in salmon can

A technology for salmon and canned food, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of ambiguous labeling of canned salmon food raw materials, and the identification and analysis technology cannot accurately identify the variety of raw materials, etc., so as to achieve simple and easy operation, Optimize the extraction method and the effect of easy operation

Inactive Publication Date: 2016-09-07
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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Problems solved by technology

[0008] The main purpose of the present invention is to develop a method for identifying the species of salmon in canned salmon using micro-barcode technology for the problem that the labeling of canned salmon food raw materials is vague and the existing identification and analysis technology cannot accurately identify the species of the raw material

Method used

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  • Method for identifying salmon variety in salmon can
  • Method for identifying salmon variety in salmon can
  • Method for identifying salmon variety in salmon can

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Experimental program
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Embodiment

[0032] 1. Sample collection Collect samples of canned salmon with different processing methods from the market, such as Kabayaki, water immersion, oil immersion, etc.

[0033] 2. DNA extraction

[0034]a) Weigh 100mg of canned salmon muscle tissue sample into a 2mL centrifuge tube, add 1mL of chloroform:methanol:water=1:2:0.8 (volume ratio), let it stand at room temperature overnight, centrifuge at 5000rpm for 3min, discard the supernatant, and remove aseptically. Wash the sample with ion water, and store the sample tissue at -40°C for later use;

[0035] b) Cut the sample obtained in step a) into pieces under sterile conditions and add 1mL of DNA extraction solution. The composition of the extraction solution is 4% CTAB, 100mmol / L Tris-HCl (pH 8.0), 1.4mol / L NaCl, 20mmol / L EDTA (pH 8.0), placed in a constant temperature water bath at 65°C, incubate for 1-2 hours, and mix well by inverting from time to time until the sample is completely digested; after incubation, centrifuge...

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Abstract

The invention discloses a method for identifying a salmon variety in a salmon can, in particular a method for identifying the salmon variety in the salmon can with the application of a micro-barcode technology; the method mainly comprises the following steps: a) weighing a certain mass of a salmon can sample and extracting DNA; b) conducting amplification by virtue of a micro-barcode primer by taking the DNA obtained in the step a) as a template, so as to obtain a DNA segment of 212bp; and c) sequencing the segment which is obtained through amplification in the step b), and conducting sequence characteristic contrast with an existing DNA database, so that information on the salmon variety is obtained. The method for identifying the salmon variety in the salmon can with the application of the micro-barcode technology established by the invention has very important practical significance to the improvement of product quality and safety levels of China's canning industry, the standardization of industry development and the protection of consumers' lawful rights and consumers' rights to know.

Description

technical field [0001] The invention discloses a method for identifying salmon species in canned salmon, in particular a method for identifying salmon species in canned salmon by applying micro-barcode technology, belonging to the technical field of food authenticity identification. Background technique [0002] Salmon is the general name of salmon and trout. It is a rare edible fish and is mainly distributed in cold water fish in high latitude areas. Because it is rich in protein and fatty acids needed by the human body, it is healthy, green and pollution-free, and has high nutritional value. Loved by the public. Salmonidae includes 3 subfamilies, 7 genera and 36 species, mainly Salmoniae, Coregoninae and Thymallidae. Codex Alimentarius Commission CAC (CODEX STAN 3-1981) "Canned Salmon" stipulates that Atlantic salmon (Salmosalar), sockeye salmon (Oncorhynchusnerka), coho salmon (Oncorhynchus kisutch), salmon (Oncorhynchustschawytscha), humpbacked horse Salmon (Oncorhynch...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2531/113C12Q2563/185
Inventor 孟镇仇凯钟其顶安丽艳杨彤辉
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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